[Histonet] Cyto Controls for PAP

Mon Nov 4 11:13:32 CST 2019

In our lab, we evaluate a slide from a case in each staining run and document the staining of the nucleus and cytoplasm that are present within the slide.  We also run a blank slide in each batch and evaluate it for any potential floaters.  Lastly, we stain our FNA specimens separately from any other non-gyn or FNA case.  We are CAP accredited these steps have fulfilled their requirements.

Joe W. Walker, Jr. MS, SCT(ASCP)
Anatomical Pathology and Interim Phlebotomy Manager
P 802.747.1790
joewalker at rrmc.org

-----Original Message-----
From: Olszewski, Dawn via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Monday, November 4, 2019 9:11 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Cyto Controls for PAP

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Good afternoon everyone,

I am now the manager over Histology and Cytology.  I know very little about Cytology, so I am hoping you guys can help me out.  We do non-gyn cyto but do not have a cytotechnologist.  Our pathologist read all the slides and the histo techs do the prep work.  We are probably going to CAP soon and one of the questions is, "how do you assess the quality of cytopathology stains"? For histology we run a H&E control down the stain line prior to staining any patient slides and evaluate it for proper staining of the nucleus and the cytoplasm.  How is this handled in cytology using the PAP stain?  If you make controls, how is it done and stored and what criteria do you use to assess the quality?  Sorry for my ignorance, but I am a histotech and not a cytotech by trade.

Dawn Olszewski HTL(ASCP)QIHC

Pathology Manager

South Georgia Medical Center

P: (229) 259-4830

E: dawn.olszewski at sgmc.org

Dawn Olszewski
Pathology Manager - Histotechnologist
Laboratory

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South Georgia Medical Center
2501 N. Patterson St.
Valdosta, GA 31602
229-259-4830 (O) | 229-560-6191 (M)
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