[Histonet] FW: Fridge temp

[Morken, Timothy]

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-----Original Message-----
From: Morken, Timothy 
Sent: Wednesday, March 27, 2019 8:30 AM
To: 'P Sicurello'; MARY ANN
Subject: RE: [Histonet] Fridge temp

You will need to retest to confirm reliability. That would be easier that buying all new -which you would then have to test anyway.

In the old, old days most antibodies were serum-based and were susceptible to degradation and bacterial growth. Modern antibodies and buffer formulations are very robust and I think you will find that few, if any, will fail. When I worked for an antibody company I used to do accelerated longevity testing by putting antibodies in a 40C oven for several MONTHS. I can't recall any failing, and some worked even better after that exposure! However,  some that have been opened and could show bacterial contamination. 

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-----Original Message-----
From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Tuesday, March 26, 2019 8:53 PM
To: MARY ANN
Cc: HistoNet
Subject: Re: [Histonet] Fridge temp

Mary Ann,

First I would call the vendor of the antibodies and detection kits and ask
them if they have any data about their reagents being warmed up for those
time periods.  If they can't provide an answer, then I suggest testing
every single one of the antibodies and detection kits to see if they still
work. I would do something like a mini-validation.  Another thing you can
do is a literature search that demonstrates the efficacy of warmed up, or
old, antibodies/detection kits.

Tally up the cost of replacing all the warmed up reagents. Then calculate
the cost of running a test that fails due to the warmed reagents - tech
time (remember to include whatever percentage the company adds in terms of
benefits), reagents used, etc.  Also include the effect on the impression
of the clients if you need to repeat a test because the first one failed
and you were aware of the reason why it failed.

Overall, you are better off replacing all of the items that were warmed up.
As for the refrigerator, install a temperature tracking system that will
notify someone if the temperature goes out of range.

If your lab is licensed by any governing agency, let the CFO/Owner know
about any rules or regulations that you must follow when it is suspected
that a reagent has gone bad, or expires.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

9300 Campus Point Drive

La Jolla, CA 92037
(P): 858-249-5610



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On Tue, Mar 26, 2019 at 4:15 PM MARY ANN via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Let's say, hypotheticaly, if you discover your fridge with all you
> antibodies and detection kits were discovered to have been at 19c. for an
> undisclosed amout of time. 12 24 48 hours due tona power surge..south
> Florida weather.
>
> Let's also propose your lab CFO/Owner dosent think its a big deal.
>
> First how would you handle the issue given the frisge has been restored ?
>
>
>
>
> Sent from Xfinity Connect Application
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