[Histonet] PAP stains

[Joe W. Walker, Jr.]

There are many reasons why the nuclei may look dark in the Pap stain.
1. What type of hematoxylin are you using?
2. What type of Pap stain are you using, regressive or progressive?

This could be due to a variety of things like: type of preparation utilized, too long in hematoxylin, not enough rinsing of excess hematoxylin, not enough time in HCL (if used), too long in bluing, etc., personal preference of interpreting cytotech or pathologist, just to name a few.

Joe W. Walker, Jr. MS, SCT(ASCP)
Anatomical Pathology Manager
joewalker at rrmc.org, www.rrmc.org

-----Original Message-----
From: Charles Riley via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Tuesday, March 19, 2019 3:18 PM
To: Histo List <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] PAP stains

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What causes dark nuclei in the PAP stain.

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Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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