[Histonet] [External] Re: SDHB IVD antibody

Tue Jun 25 14:16:13 CDT 2019

We use the antibody at a dilution of 1:50 following low-pH HIER (20 minutes) on the Leica Bond Max IHC platform.  Our primary antibody incubation is 15 minutes.  Detection is Bond Refine HRP with DAB. 

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
Richard.cartun at hhchealth.org

-----Original Message-----
From: Bitting, Angela K. [mailto:akbitting at geisinger.edu] 
Sent: Tuesday, June 25, 2019 1:03 PM
To: Cartun, Richard
Subject: RE: [External] Re: [Histonet] SDHB IVD antibody

EXTERNAL email from Outside HHC! Do NOT open attachments or click links from unknown senders.

Would you share your procedure?

-----Original Message-----
From: Cartun, Richard via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Tuesday, June 25, 2019 12:31 PM
To: Emmanuel Geoffrey S Gonzaga
Cc: histonet at lists.utsouthwestern.edu
Subject: [External] Re: [Histonet] SDHB IVD antibody

Cell Marque offers an excellent SDHB rabbit monoclonal (clone EP288) that we use in our laboratory.  However, it is not an "IVD".

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
Richard.cartun at hhchealth.org

-----Original Message-----
From: Emmanuel Geoffrey S Gonzaga via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Tuesday, June 25, 2019 11:45 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] SDHB IVD antibody

EXTERNAL email from Outside HHC! Do NOT open attachments or click links from unknown senders.

Hello Histonet!

Does anyone know of a vendor that offers SDHB IVD antibody? It looks like Zeta corp. use to offer it, but has since discontinued production.

Thanks

Emmanuel Gonzaga

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