[Histonet] IHC mouse optomization

[Blanca Lopez]

Morning,
I need a good advice in optimizing Mouse antibodies for research purposes. I use Dako/Agilent products. COX6B2 from Sigma is giving us hard times. Dako kit HRP has a mixture of mouse and rabbit so we think that might be the reason become negative.
Is there any tips for optimizing antibodies special for mouse tissue? Or if you can share your best procedures in IHC mouse stains. I can have a different ways and products to try. Any suggestion or opinions count. If you have any website that I can learn more about IHC for research mainly done in  Xenograft.
Thank you everybody:)

Blanca Lopez HT (ASCP)cm
Senior Histotechnologist
UT Southwestern Medical Center
Harold C. Simmons Comprehensive Cancer Center
UTSTR Biorepository Tissue Lab
6000 Harry Hines Blvd NB5.102
Dallas, Texas 75390
214-648-7598
blanca.lopez at utsouthwestern.edu<mailto:blanca.lopez at utsouthwestern.edu>



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