[Histonet] CD27

Katy Milne kmilne at bccrc.ca
Tue Jul 30 12:19:54 CDT 2019 

We use this RbMAb from Abcam...
https://www.abcam.com/cd27-antibody-epr8569-ab131254.html


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Sent: July-30-19 10:00 AM
To: histonet at lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 188, Issue 23

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Today's Topics:

   1. CD27 (Victoria Baker)
   2. Re: Victoria blue for lung tissue
      (Dr. Michael Gudo (Morphisto GmbH))


----------------------------------------------------------------------

Message: 1
Date: Mon, 29 Jul 2019 21:45:40 -0400
From: Victoria Baker <bakevictoria at gmail.com>
To: Histo Net list server <HistoNet at lists.utsouthwestern.edu>
Subject: [Histonet] CD27
Message-ID:
	<CALYZRmQ7pORbfipvJVmNqYK7a1xh+jfXVOqHux3h=qWFNQx8kg at mail.gmail.com>
Content-Type: text/plain; charset="UTF-8"

Hi!

Is anyone currently using CD27 on FFPE human tissue?

I found one source and it is for lyophilized CD27.  I'd like to find one that is a concentrate.  I have given up on a pre-dilute.  I would like to see if there is both a mono and a poly clonal ab available and is I'm use.

Thank you in advance for your help.

Vikki

Thank


------------------------------

Message: 2
Date: Tue, 30 Jul 2019 13:01:02 +0200
From: "Dr. Michael Gudo (Morphisto GmbH)" <michael.gudo at morphisto.de>
To: "Tony Henwood (SCHN)" <tony.henwood at health.nsw.gov.au>
Cc: Bob Richmond <rsrichmond at gmail.com>,	Histonet
	<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Victoria blue for lung tissue
Message-ID: <122E361B-D386-4AF6-A8CC-DDB4377A3BE8 at morphisto.de>
Content-Type: text/plain;	charset=utf-8

There is also a Kit and the ready to use solution available from MORPHISTO Germany.

Just contact the sales office: info at morphisto.de

Cheers
Michael



> Am 27.07.2019 um 23:53 schrieb Tony Henwood (SCHN) via Histonet <histonet at lists.utsouthwestern.edu>:
> 
> Methods found here:
> 
> https://www.ihcworld.com/_protocols/special_stains/miller's_elastic_el
> lis.htm
> 
> http://www.histosearch.com/histonet/Dec98/Millersstainforelasticfib.ht
> ml
> 
> https://www.sakuraus.com/getattachment/774d89be-4b32-4d69-bb02-8dea75c
> 52c0c/858
> 
> ?
> 
> 
> 
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
> Principal Scientist, the Children's Hospital at Westmead Adjunct 
> Fellow, School of Medicine, University of Western Sydney
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> Pathology Department
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, 
> Westmead NSW 2145, AUSTRALIA ________________________________
> From: Bob Richmond <rsrichmond at gmail.com>
> Sent: Saturday, July 27, 2019 11:27:54 AM
> To: Tony Henwood (SCHN)
> Subject: Re: [Histonet] Victoria blue for lung tissue
> 
> Is this method published anywhere that "Amy" would likely have access to? This looked to me like one of those cases of the blind leading the blind that are all to common in research involving histochemistry.
> 
> On Fri, Jul 26, 2019 at 8:44 PM Tony Henwood (SCHN) <tony.henwood at health.nsw.gov.au<mailto:tony.henwood at health.nsw.gov.au>> wrote:
> Victoria Blue is the dye used in Miller's Stain for Elastic Tissue.
> It is also used in the Roche Ventana Benchmark Stainer to stain elastic Tissue:
> 
> Miller PJ (1971) An elastin stain. Med Lab Technol 28, 148-149
> 
> Karen Percival & Zaher Radi (2017) Comparison of five elastin 
> histochemical stains to identify pulmonary small vasculature, Journal 
> of Histotechnology, 40:3, 73-78
> 
> Yufeng Yu & Clifford M. Chapman (2000) "Elastic Tissue Staining in 
> Human Skin" Histologic 32(1): 12
> 
> Roten SV, Bhat S, Bhawan J. Elastic fibers in scar tissue. J Cutan Pathol 1996: 23: 37-42.
> 
> 
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
> Principal Scientist, the Children's Hospital at Westmead Adjunct 
> Fellow, School of Medicine, University of Western Sydney
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> Pathology Department
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, 
> Westmead NSW 2145, AUSTRALIA
> 
> ________________________________________
> From: Bob Richmond via Histonet 
> <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwester
> n.edu>>
> Sent: Saturday, July 27, 2019 4:10 AM
> To: 
> Histonet at lists.utsouthwestern.edu<mailto:Histonet at lists.utsouthwestern
> .edu>
> Subject: Re: [Histonet] Victoria blue for lung tissue
> 
> Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent 
> FFPE lung tissue to exam thickness of artery. Could anybody recommend 
> a good vendor of this reagent kit?<<
> 
> You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich 
> and several others. I couldn't find anyone who offers a kit, only the dry dye.
> There are other dyes called Victoria Blue, reported to give the same 
> results.
> 
> You'd have to find a method for preparing it as an elastic stain, and 
> I couldn't find such a method either with Google or in my old books. 
> The requester needs to supply you with a method. I suspect the 
> requester is reading an old article. There are stains for elastic 
> tissue (which is I suppose what you want to "exam thickness of 
> artery") that are a lot easier to get.
> 
> Bob Richmond
> Samurai Pathologist
> Maryville TN
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