[Histonet] Derm IHC question

[Cartun, Richard]

Doesn't sound like a fixation issue to me.  Could the tissue be drying out before it's placed in formalin?  Also, are these specimens inked for assessment of margins?  I've seen ink interfere with immunoreactivity.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
Richard.cartun at hhchealth.org

-----Original Message-----
From: Debra Siena via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Friday, February 08, 2019 11:51 AM
To: 'histonet'
Subject: [Histonet] Derm IHC question

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Hello fellow Histonetters

I would like to ask you a question about IHC staining and derm cases.  I am seeing a peculiar issue going on, where the melanocytes in the middle of the tissues are staining pretty well but when you get to the ends of the tissues either shaves or ellipses, they are not staining. This is sporadic, not every case and there is no consensus as to a common thread between the cases.    I feel that this may be a fixation issue but was just wondering if anyone had ever seen the same phenomena and would be willing to share the theory or even better what was the remedy behind this issue.  The fixative is 10% Neutral Buffered Formaliln  and the cells in question that are "dropping out" which is what the pathologist is describing are melanocytes, especially with Sox-10 and Mart 1 antibodies.

Thanks for the assistance, I definitely appreciate it very much.



Best wishes,



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