[Histonet] plural fluid prep

[Beth Cox]

Hi Michelle,

When I was in my Cytology training, we did an experiment to answer just 
that question.  We started with a 2 liter jug of plural fluid with 
nothing added, and we stored in in the fridge.  At first we tested it 
every day to see if there was any degeneration, then we got tired of 
that and tested twice a week, then once a week. After 4 months it was 
still just fine, and we threw it away because we were tired of testing 
it (and it was the end of the semester!).   It seems that the cells are 
happy in their natural habitat and refrigeration slowed/prevented any 
bacterial growth.

So  my advice is:  don't add anything to it, keep it in the fridge, and 
it will last many months!!

Beth Cox, SCT/HTL(ASCP)QIHC

From: Michelle Jamison<m.jamison at elitechgroup.com>
To:"histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] plural fluid prep
Message-ID:<d4756630-95cf-220b-00bb-d9c9ab50bed5 at elitechgroup.com>
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Hi All,
I work in a research lab where we are using plural fluid to proof a
method. Recently we have received some, and are unsure how long it will
be ok in the fridge, or if we should add some preservation fluid to it.
Can anyone advise please.