[Histonet] Delay in embedding
Terri Braud
tbraud at holyredeemer.com
Thu Dec 19 12:11:27 CST 2019
We do it all the time.
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
-----Original Message-----
From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu]
Sent: Wednesday, December 18, 2019 1:00 PM
To: histonet at lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 193, Issue 12
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Today's Topics:
1. Delay in embedding? (P Sicurello)
2. Re: Delay in embedding? (Theresa Dalton)
3. Re: Delay in embedding? (Tony Henwood (SCHN))
4. Re: Delay in embedding? (Jennifer Saunders)
5. Re: Delay in embedding? (Garrey Faller)
6. Happy Holidays Histonetters! Here are the 12 days of
Christmas for histotechs by Ashley Troutman. Enjoy! (Pam Barker)
7. plural fluid prep (Michelle Jamison)
----------------------------------------------------------------------
Message: 1
Date: Tue, 17 Dec 2019 11:30:58 -0800
From: P Sicurello <patpxs at gmail.com>
To: HistoNet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Delay in embedding?
Message-ID:
<CAPSjddacHtoHxwmgHzqY_jF+1r=SExBHUwV0uK=ui3Zf0_1p6A at mail.gmail.com>
Content-Type: text/plain; charset="UTF-8"
Good Morning Listers,
How many out there will process tissue and then leave the cassettes at room
temperature and embed it at a later time (hours or the next day)?
Please send me your opinions about doing this. I think it?s a bad idea,
others I speak with disagree.
Sincerely,
Paula Sicurello, HTL (ASCP)CM
Histotechnology Specialist
UC San Diego Health
9300 Campus Point Drive
La Jolla, CA 92037
(P): 858-249-5610
*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material. Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited. If you received this e-mail in error,
please contact the sender and delete the material from any computer.
------------------------------
Message: 2
Date: Tue, 17 Dec 2019 15:33:43 -0500
From: Theresa Dalton <terrie.dalton at icloud.com>
To: P Sicurello <patpxs at gmail.com>
Cc: HistoNet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Delay in embedding?
Message-ID: <693B4D62-DF63-486C-9017-D24B9F190136 at icloud.com>
Content-Type: text/plain; charset=utf-8
We have done this - only on an emergency basis.
Sent from my iPhone
> On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>
> ?Good Morning Listers,
>
>
>
> How many out there will process tissue and then leave the cassettes at room
> temperature and embed it at a later time (hours or the next day)?
>
>
>
> Please send me your opinions about doing this. I think it?s a bad idea,
> others I speak with disagree.
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 9300 Campus Point Drive
>
> La Jolla, CA 92037
> (P): 858-249-5610
>
>
>
> *Confidentiality Notice*: The information transmitted in this e-mail is
> intended only for the person or entity to which it is addressed and may
> contain confidential and/or privileged material. Any review,
> retransmission, dissemination or other use of or taking of any action in
> reliance upon this information by persons or entities other than the
> intended recipient is prohibited. If you received this e-mail in error,
> please contact the sender and delete the material from any computer.
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Tue, 17 Dec 2019 22:28:13 +0000
From: "Tony Henwood (SCHN)" <tony.henwood at health.nsw.gov.au>
To: Theresa Dalton <terrie.dalton at icloud.com>, P Sicurello
<patpxs at gmail.com>
Cc: "'histonet at lists.utsouthwestern.edu'"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Delay in embedding?
Message-ID:
<e97666e5e1ab4aef946b83e65f8b40ec at SVDCMBX-MEX024.nswhealth.net>
Content-Type: text/plain; charset="utf-8"
Hi Paula,
We routinely do this, especially for our fetal autopsy blocks.
We are then able to process and let them set at room temp until we are able to embed and cut.
Some cases are more urgent than others so these can be expedited a lot easier since they will only need embedding, sectioning and staining. We also get best usage of our limited processing capabilities.
It is more efficient for us.
Morphology, staining and immunohistochemistry is not affected.
It is better to do this rather than leave them at 64oC plus for extended times prior to embedding since many antigens will be adversely affected.
Take home point - do not overcook your tissues.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children?s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: Theresa Dalton via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, 18 December 2019 7:34 AM
To: P Sicurello <patpxs at gmail.com>
Cc: HistoNet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Delay in embedding?
We have done this - only on an emergency basis.
Sent from my iPhone
> On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>
> ?Good Morning Listers,
>
>
>
> How many out there will process tissue and then leave the cassettes at
> room temperature and embed it at a later time (hours or the next day)?
>
>
>
> Please send me your opinions about doing this. I think it?s a bad
> idea, others I speak with disagree.
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 9300 Campus Point Drive
>
> La Jolla, CA 92037
> (P): 858-249-5610
>
>
>
> *Confidentiality Notice*: The information transmitted in this e-mail
> is intended only for the person or entity to which it is addressed and
> may contain confidential and/or privileged material. Any review,
> retransmission, dissemination or other use of or taking of any action
> in reliance upon this information by persons or entities other than
> the intended recipient is prohibited. If you received this e-mail in
> error, please contact the sender and delete the material from any computer.
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender.
Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities.
------------------------------
Message: 4
Date: Wed, 18 Dec 2019 07:07:35 +0000 (UTC)
From: Jennifer Saunders <jsaundersht at yahoo.com>
To: "Tony Henwood (SCHN)" <tony.henwood at health.nsw.gov.au>, Theresa
Dalton <terrie.dalton at icloud.com>, P Sicurello <patpxs at gmail.com>
Cc: "'histonet at lists.utsouthwestern.edu'"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Delay in embedding?
Message-ID: <920732173.736881.1576652855323 at mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8
I don?t see a problem with it. We all melt and re-embed tissue for all kinds of reasons every day. I think it is much better for the tissue than keeping it warm for an extended period of time.?Jen Saunders?
Sent from Yahoo Mail for iPhone
On Tuesday, December 17, 2019, 2:28 PM, Tony Henwood (SCHN) via Histonet <histonet at lists.utsouthwestern.edu> wrote:
Hi Paula,
We routinely do this, especially for our fetal autopsy blocks.
We are then able to process and let them set at room temp until we are able to embed and cut.
Some cases are more urgent than others so these can be expedited a lot easier since they will only need embedding, sectioning and staining. We also get best usage of our limited processing capabilities.
It is more efficient for us.
Morphology, staining and immunohistochemistry is not affected.
It is better to do this rather than leave them at 64oC plus for extended times prior to embedding since many antigens will be adversely affected.
Take home point - do not overcook your tissues.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children?s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: Theresa Dalton via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Wednesday, 18 December 2019 7:34 AM
To: P Sicurello <patpxs at gmail.com>
Cc: HistoNet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Delay in embedding?
We have done this - only on an emergency basis.
Sent from my iPhone
> On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>
> ?Good Morning Listers,
>
>
>
> How many out there will process tissue and then leave the cassettes at
> room temperature and embed it at a later time (hours or the next day)?
>
>
>
> Please send me your opinions about doing this.? I think it?s a bad
> idea, others I speak with disagree.
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 9300 Campus Point Drive
>
> La Jolla, CA 92037
> (P): 858-249-5610
>
>
>
> *Confidentiality Notice*: The information transmitted in this e-mail
> is intended only for the person or entity to which it is addressed and
> may contain confidential and/or privileged material.? Any review,
> retransmission, dissemination or other use of or taking of any action
> in reliance upon this information by persons or entities other than
> the intended recipient is prohibited.? If you received this e-mail in
> error, please contact the sender and delete the material from any computer.
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender.
Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities.
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Wed, 18 Dec 2019 05:09:23 -0500
From: Garrey Faller <garreyf at gmail.com>
To: Jennifer Saunders <jsaundersht at yahoo.com>
Cc: "Tony Henwood (SCHN)" <tony.henwood at health.nsw.gov.au>, Theresa
Dalton <terrie.dalton at icloud.com>, P Sicurello <patpxs at gmail.com>,
"histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Delay in embedding?
Message-ID: <31C3BB02-24D4-4C02-ABA5-C744B4FE74E9 at gmail.com>
Content-Type: text/plain; charset=utf-8
I?ve done it a handful of times when my only Histotech called in sick.
No effect on quality.
Garrey
Sent from my iPhone
> On Dec 18, 2019, at 2:24 AM, Jennifer Saunders via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>
> ?I don?t see a problem with it. We all melt and re-embed tissue for all kinds of reasons every day. I think it is much better for the tissue than keeping it warm for an extended period of time. Jen Saunders
>
>
> Sent from Yahoo Mail for iPhone
>
>
> On Tuesday, December 17, 2019, 2:28 PM, Tony Henwood (SCHN) via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>
> Hi Paula,
>
> We routinely do this, especially for our fetal autopsy blocks.
> We are then able to process and let them set at room temp until we are able to embed and cut.
> Some cases are more urgent than others so these can be expedited a lot easier since they will only need embedding, sectioning and staining. We also get best usage of our limited processing capabilities.
> It is more efficient for us.
>
> Morphology, staining and immunohistochemistry is not affected.
>
> It is better to do this rather than leave them at 64oC plus for extended times prior to embedding since many antigens will be adversely affected.
>
> Take home point - do not overcook your tissues.
>
>
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Principal Scientist, the Children?s Hospital at Westmead
> Adjunct Fellow, School of Medicine, University of Western Sydney
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> Pathology Department
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
>
> -----Original Message-----
> From: Theresa Dalton via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Sent: Wednesday, 18 December 2019 7:34 AM
> To: P Sicurello <patpxs at gmail.com>
> Cc: HistoNet <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Delay in embedding?
>
> We have done this - only on an emergency basis.
>
> Sent from my iPhone
>
>> On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>>
>> ?Good Morning Listers,
>>
>>
>>
>> How many out there will process tissue and then leave the cassettes at
>> room temperature and embed it at a later time (hours or the next day)?
>>
>>
>>
>> Please send me your opinions about doing this. I think it?s a bad
>> idea, others I speak with disagree.
>>
>> Sincerely,
>>
>> Paula Sicurello, HTL (ASCP)CM
>>
>> Histotechnology Specialist
>>
>> UC San Diego Health
>>
>> 9300 Campus Point Drive
>>
>> La Jolla, CA 92037
>> (P): 858-249-5610
>>
>>
>>
>> *Confidentiality Notice*: The information transmitted in this e-mail
>> is intended only for the person or entity to which it is addressed and
>> may contain confidential and/or privileged material. Any review,
>> retransmission, dissemination or other use of or taking of any action
>> in reliance upon this information by persons or entities other than
>> the intended recipient is prohibited. If you received this e-mail in
>> error, please contact the sender and delete the material from any computer.
>> _______________________________________________
>> Histonet mailing list
>> Histonet at lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender.
>
> Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities.
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Wed, 18 Dec 2019 11:32:13 -0500
From: "Pam Barker" <relia1 at earthlink.net>
To: "Histopeeps Histonet" <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Happy Holidays Histonetters! Here are the 12 days
of Christmas for histotechs by Ashley Troutman. Enjoy!
Message-ID: <0a5001d5b5c0$b402ac40$1c0804c0$@earthlink.net>
Content-Type: text/plain; charset="iso-8859-1"
Hi Histonetters,
Happy Holidays!
Here is a gem posted on the histonet in 12/2013
The 12 days of Christmas for Histotechs !
written by Ashley Troutman
?Hello all,
I hope the holidays are treating everyone well. (Especially in light of us
having the most hazardous job in the country...) Here's some fun for the
season:
The Twelve Days of Histo
TWELVE Medical Directors
ELEVEN logs-a-printing
TEN residents reading
NINE frozen sections
EIGHT administrators
SEVEN stainers beeping
SIX techs complaining
FIVE I...H...Cs.... :)
FOUR calling docs
THREE recuts
TWO special stains
And a block with an H and EEEEE...
Please feel free to sing along.
Merry Christmas in Histoland!?
Ashley Troutman BS, MBA, HT(ASCP) QIHC
Histonetters,
I have some REALLY Exciting opportunities,
Here?s a quick list:
Leadership:
MA - Field Applications Specialist ? IHC- Boston
IL - Field Applications Specialist ? IHC ? Chicago
MO - Histology Lab Manager ? St. Louis
TN - Histology Supervisor - Nashville
Histotechnicians/Histotechnologists
North Carolina
South Carolina
Wisconsin
Florida
Kansas
New York
California
I have new opportunities coming in all of the time. Let me know if anything
looks interesting on my list or if there is a specific area you would like
for me to be on the lookout for.
We can start chatting now about opportunities after the new year!!
The early bird gets the worm!!
I also can help if you are short staffed!
I can be reached asap on my cell/text at 407-353-5070 or email me at
relia1 at earthlink.net
Happy Holidays to you and yours!!!!
Happy Holidays !!!!
Thanks-Pam
#jobs4myhistopeeps
#ilovemyhistopeeps
#histopeeps
Follow my hashtags and make your day great and your career greater!!
Right Place, Right Time, Right Move with RELIA!
Thank You!
?Pam M. Barker?
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:???? (407)353-5070
FAX:???? (407)678-2788
E-mail: relia1 at earthlink.net
https://www.facebook.com/RELIASolutionsforhistologyprofessionals
www.facebook.com/PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia
------------------------------
Message: 7
Date: Wed, 18 Dec 2019 10:51:40 -0700
From: Michelle Jamison <m.jamison at elitechgroup.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] plural fluid prep
Message-ID: <d4756630-95cf-220b-00bb-d9c9ab50bed5 at elitechgroup.com>
Content-Type: text/plain; charset=utf-8; format=flowed
Hi All,
I work in a research lab where we are using plural fluid to proof a
method. Recently we have received some, and are unsure how long it will
be ok in the fridge, or if we should add some preservation fluid to it.
Can anyone advise please.
Thank you in advance, and Happy Holidays to all!!
--
Best of all things to you,
Michelle Jamison
//
Tel : +1.435.752.6011 Ext. 435.227.1474
m.jamison at elitechgroup.com <mailto:> ? www.elitechgroup.com
<http://www.elitechgroup.com/>
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