[Histonet] Xylene substitutes for clearing agents

[Rene J Buesa]

 Under separate cover I am sending you 3 papers of mine that answer your question.René
    On Wednesday, August 28, 2019, 08:45:31 AM EDT, Ingles Claire via Histonet <histonet at lists.utsouthwestern.edu> wrote:  
 
 Chris:
Propar from Anatech works great for us. I believe it is still advisable to use xylene in the cleaning cycle on the processors though. We had to go back the other way a bit when our Doc wanted a tape coverslipper. Now he gripes about the xylene smell. Hmmm.
Claire

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From: Hagon, Christopher (Health) via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Tuesday, August 27, 2019 10:53 PM
To: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Xylene substitutes for clearing agents

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Hello histonetters!

I realise that this has been asked a lot, but cannot find a good link for the comparisons of each. I am charged with looking into converting our lab to go xylene free. We don't want to go down the limonene path, so that leaves the isopropyl alcohol method, or the aliphatic hydrocarbon xylene substitution (Leica Sub-X etc).

Looking for opinions from each camp if possible, on how easy or hard it was to change procedures. Was there much trial and error in changing the processing protocols with the aliphatics? Any pitfalls I should look out for?

Any input greatly appreciated.


Chris Hagon | Senior Scientist, Anatomical Pathology
ACT Pathology | health.act.gov.au<https://health.act.gov.au/>



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