[Histonet] Xylene substitutes for clearing agents

[Hagon, Christopher (Health)]

UNOFFICIAL

Hello histonetters!

I realise that this has been asked a lot, but cannot find a good link for the comparisons of each. I am charged with looking into converting our lab to go xylene free. We don't want to go down the limonene path, so that leaves the isopropyl alcohol method, or the aliphatic hydrocarbon xylene substitution (Leica Sub-X etc).

Looking for opinions from each camp if possible, on how easy or hard it was to change procedures. Was there much trial and error in changing the processing protocols with the aliphatics? Any pitfalls I should look out for?

Any input greatly appreciated.


Chris Hagon | Senior Scientist, Anatomical Pathology
ACT Pathology | health.act.gov.au<https://health.act.gov.au/>



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