[Histonet] tissue blocks stored in cooler C for 2wks - any successwith IHC?

[Sheeder, Christopher]

Totally agree with Brett & Colleen.
It is very tempting to have an abundant source of study tissue but the results won't be worth the investment.
Best of luck.
Chris


Christopher Sheeder, HT(ASCP)QIHC
Histology Supervisor | Department of Laboratories
Seattle Children’s Hospital
4800 Sand Point Way NE
Seattle, WA 98105

Office: 206-987-6259

christopher.sheeder at seattlechildrens.org


-----Original Message-----
From: Colleen Forster <cforster at umn.edu>
Sent: Thursday, August 22, 2019 11:27 AM
To: Brett Connolly <brettmc31 at comcast.net>
Cc: M.O. <modz9636 at gmail.com>; Histonet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] tissue blocks stored in cooler C for 2wks - any successwith IHC?

I agree with Brett.

I believe the histological aspect will be horrible and probably not worth the time and money spent. I feel you would be terribly disappointed with the lack of usable results.

Colleen Forster HT(ASCP)QIHC

On Thu, Aug 22, 2019 at 11:14 AM Brett Connolly via Histonet < histonet at lists.utsouthwestern.edu> wrote:

> Merissa,
> I have done tons of IHC assays on frozen tissues, including many
> procured from commercial tissue banks. My experience has been that the
> most of the time the microscopic morphology is terrible (abysmal if it
> is brain tissue). Cells are always blown open from slow freezing. It’s
> bad enough that there is a 2 week PMI…add to that storage at 4C
> instead of at least -20C (best would be -80C) and IMO it’s a recipe
> for failure. Wouldn’t touch that tissue no way, no how…well maybe if
> they paid ME I would take it and try to use it for autoradiography or something.
> Just my opinion.
>
> Brett Connolly HTL (ASCP), PhD
>
> Sent from Mail for Windows 10
>
> From: M.O. via Histonet
> Sent: Thursday, August 22, 2019 11:24 AM
> To: Histonet
> Subject: [Histonet] tissue blocks stored in cooler C for 2wks - any
> successwith IHC?
>
> Good morning!
>
> We are working with a tissue bank that is only able to procure tissue
> blocks from donors that have postmortem intervals of 2wks or more. The
> storage conditions are in a cooler (I believe 4C), the block will be
> procured and frozen and we will harvest the tissue from that block
> once we thaw it. We plan to use these samples for histological purposes only.
>
> Has anyone had success with IHC on donors/tissues that were stored in
> 4C for 2wks or more? Will the integrity of the cells and proteins be okay?
>
> Thank you,
> Merissa
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--
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
B173 PWB      612-626-1930

*If submitting histology request please also forward to Lori Holm at holml at umn.edu <holml at umn.edu>*

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