[Histonet] different dehydration methods after staining with PAP

Michelle Jamison m.jamison at elitechgroup.com
Wed Aug 14 14:23:44 CDT 2019


Hi All with experience with PAP staining,
In the last steps of PAP staining there are dehydration steps (with 
alcohol) then coversliping. Do you ever dehydrate with air, or always 
with alcohol? Also, do you ever have a problem with moisture being 
caught under the coverslip causing a cloudy effect? If so, what do you 
do for that? Last one....if you do dehydrate with alcohol, would you 
ever consider air dehydration instead?
Thanks for your input,
Cheers!
Michelle
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Best of all things to you,

Michelle Jamison

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Tel : +1.435.752.6011 Ext. 435.227.1474

m.jamison at elitechgroup.com <mailto:> • www.elitechgroup.com 
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