[Histonet] biopsy staining

[Hobbs, Carl]

Hi
Are you processing to Pwax?
If so, I stain my mouse DRGS ( very small) using Haemalum.
5 mins max ( I use Gill's I) then blue in alkaline tapwater for 10 mins before Pwax processing
Stain from Formalin or alcohol but.....rinse in dist water to remove before staining.
The "blue" stays nicely in the tissues, for embedding : sure, I then subject the sections to HIER without pretreatment as the citric acid treatment gets rid of any dye.
If doing dye-staining I will acid-alcohol treat the sections before staining.
Works well for me.

Good luck 

Carl 
 
Carl Hobbs FIBMS 
Histology and Imaging Manager 
Wolfson CARD 
Guys Campus, London Bridge  
Kings College London 
London 
SE1 1UL 
  
020 7848 6813