[Histonet] Luxol fast Blue Question

[Cooper, Brian]

Hi Lisa, 

We keep them in solution at 56-60 degrees overnight, and typically start differentiation between the 15-20 hour range.     

Thanks, 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcooper at chla.usc.edu 


-----Original Message-----
From: Chapman, Lisa via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, September 12, 2018 12:18 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Luxol fast Blue Question (EXTERNAL EMAIL)

Hello All,

When performing a LFB for myelin and nissl, how long does everyone keep their slides in the LFB solution for and what temp do you use?  Most procedures say "overnight" and Sheehan states 16-24 hours.

Any advice would be greatly appreciated!  Thank you in advance!

Lisa Chapman HT (ASCP)
ACL Laboratories
Milwaukee, WI
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