[Histonet] Platelet staining on FFPE mouse tissues

[Ana Maluenda]

Hi everyone,

Does anyone has experience performing IHC staining for platelets on mouse FFPE?

I've been trying for a while with different primary antibodies unsuccessfully. I found two that work in cryosections, but not a single hint of positivity on FFPE tissues. The samples have been prepared as usual (NBF fixation, graded EtOH, Xylene, wax...) and thin sections were collected by usual procedures (think they are 5 um). Unfortunately I have limited resources, so my protocol has been manual staining (no autostainer) and I have only microwave or water baths available around to play with Ag retrievals. I also already tried Sodium Citrate and Citrate buffers at pH = 6 and 9. Anyone has a protocol that works and that they would be willing to share?

Much appreciated!

Kind regards,

Ana
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