[Histonet] Kwik Diff
Anne van Binsbergen
annigyg at gmail.com
Fri Sep 7 13:12:49 CDT 2018
Hi Histonetters
Try the Kwik Diff from Thermo Shandon. We have been using it for as long as I can remember. I’m sure there must be suppliers in the USA.
Annie in Arabia
Sent from my iPhone
> On 07 Sep 2018, at 18:00, histonet-request at lists.utsouthwestern.edu wrote:
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> Today's Topics:
>
> 1. Re: Pregnancy guide for working in histology (Rene J Buesa)
> 2. Re: Pregnancy guide (Terri Braud)
> 3. Re: Giemsa vs Diff Quik (Bob Richmond)
> 4. Paraffin Dispenser (Laurie Colbert)
> 5. Re: Pregnancy guide for working in histology (Katelin Tellechea)
> 6. Re: Elephant Tissues (Histology)
> 7. Re: Elephant Tissues (Dr. Michael Gudo (Morphisto GmbH))
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 6 Sep 2018 17:21:00 +0000 (UTC)
> From: Rene J Buesa <rjbuesa at yahoo.com>
> To: Carol Torrence <ctorrence at kmcpa.com>,
> "histonet at lists.utsouthwestern.edu"
> <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Pregnancy guide for working in histology
> Message-ID: <2112362188.586296.1536254460516 at mail.yahoo.com>
> Content-Type: text/plain; charset=UTF-8
>
> Both formalin and xylene (and any other dangerous fumes) have to be avoided during pregnancy BUT if you have an efficient fumes hood to do grossing, then you should monitor exposure. Somebody NOT pregnant should gross with a personal formalin badge and, depending on the exposure result, then you may allow the pregnant employee to do grossing or not.Ren?
>
> On Thursday, September 6, 2018 1:08 PM, Carol Torrence via Histonet <histonet at lists.utsouthwestern.edu> wrote:
>
>
> Good morning!
>
> Could some of you chime in on guidelines you go by for those employee that are expecting a baby.? I have removed employee from xylene exposure during staining and cover slipping but am on the fence about grossing.? At this time the employee has been removed from grossing.? All grossing is done under an exhaust hood.? Our exposure badges have always read well below limits.? Thanks in advance!
>
> Carol M. Torrence, HT(ASCP)
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
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>
>
>
> ------------------------------
>
> Message: 2
> Date: Thu, 6 Sep 2018 17:52:36 +0000
> From: "Terri Braud" <tbraud at holyredeemer.com>
> To: "'histonet at lists.utsouthwestern.edu'"
> <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Pregnancy guide
> Message-ID:
> <48E053DDF6CE074DB6A7414BA05403F8ECF6EA44 at HRHEX02-HOS.holyredeemer.local>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Remember that contamination can also be by skin absorption. With that in mind, we provide thick Nitrile gloves with instruction to change them at least every 30 minutes, or less if they show any signs of deterioration. We have great ventilation and beyond that, when we wear our fume badges, we perform the worst exposure tasks, so when calculated, the exposure shows what it would be like if we did that task for 8 hours a day - and we STILL are way under the limit. The result: No restrictions of duties - 5 babies born to employees here without a single problem in 12 years.
> However, with that said, I would never ask a pregnant technician to hand coverslip more than the occasional slide.
> Just my 2cents - T
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
>
> 2. Pregnancy guide for working in histology (Carol Torrence)
> Message: 2
> Date: Thu, 6 Sep 2018 16:43:47 +0000
> From: Carol Torrence <ctorrence at kmcpa.com>
> To: "histonet at lists.utsouthwestern.edu"
> <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Pregnancy guide for working in histology
> Message-ID:
> <EC9DF87FECB7D34BB130455F819825FB78548618 at KMC-EXCH01.kmcpa.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Good morning!
>
> Could some of you chime in on guidelines you go by for those employee that are expecting a baby. I have removed employee from xylene exposure during staining and cover slipping but am on the fence about grossing. At this time the employee has been removed from grossing. All grossing is done under an exhaust hood. Our exposure badges have always read well below limits. Thanks in advance!
>
> Carol M. Torrence, HT(ASCP)
>
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Thu, 6 Sep 2018 16:37:43 -0400
> From: Bob Richmond <rsrichmond at gmail.com>
> To: "Histonet at lists.utsouthwestern.edu"
> <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Giemsa vs Diff Quik
> Message-ID:
> <CAOKsRH7z0yujwu=Vq+Bs1A9q2xR=V4Hvzrt9XmGkkbx03ZjXcQ at mail.gmail.com>
> Content-Type: text/plain; charset="UTF-8"
>
> Joseph A. Esposito at McClain Laboratories on Long Island asks:
>
>>> The laboratory I work at has been using the Diff Quik for years now as a
> stain for fine needle aspirates. Recently, when we tried to reorder a Diff
> Quik stain kit from our usual suppliers, we have found it to be on
> backorder. This has caused us to begin to consider the Giemsa stain as an
> alternative to the Diff Quik. Does anyone have any experience with using
> both stains and how they compare to each other? Would the Giemsa be a
> suitable alternative to replace the Diff Quik?<<
>
> Diff-Quik? (spelt like that!) is an old registered trademark for a rapid
> two-step Romanowsky stain. A number of Web sites inform me today that this
> trade name has been discontinued, I suppose because the name has been so
> commonly used generically.
>
> There have long been a number of generic fast two-step Romanowsky stains
> available, with a xanthene dye (presumably eosin Y) as solution 1, and a
> proprietary mixture of thiazine dyes as solution 2. In my experience with
> at least five of these, they all worked pretty much indistinguishably from
> brand-name Diff-Quik.
>
> There are a number of stains called Giemsa, also Romanowsky stains, but
> often slower than the "quick" stains. There is probably no advantage to
> using them for any reason.
>
> In my eightieth year, I remember with nostalgia the old Wolbach Giemsa
> technique for tissue sections. Tissue must be fixed in a dichromate
> fixative, traditionally Zenker/Helly, sections stained in four successive
> Giemsa baths, the last one overnight, and differentiated with 10%
> colophonium rosin in alcohol with microscopic control. (I've actually done
> this stain myself.)
>
> Bob Richmond
> Samurai Pathologist
> Maryville TN
>
>
> ------------------------------
>
> Message: 4
> Date: Thu, 6 Sep 2018 22:23:34 -0400
> From: Laurie Colbert <boznpl at aol.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Paraffin Dispenser
> Message-ID: <165b1d77c6c-1eb8-2d60 at webjasstg-vab23.srv.aolmail.net>
> Content-Type: text/plain; charset=utf-8
>
> I'm looking for a 2.5 gallon (approx) paraffin dispenser ASAP - new or refurbished.? I need to find one that is in stock and can be shipped immediately.? Vendors are welcome to respond.
>
> Thank you,
> Laurie Redmond
> Path MD?
> Los Angeles, CA
>
> ------------------------------
>
> Message: 5
> Date: Thu, 6 Sep 2018 20:15:49 -0700
> From: Katelin Tellechea <katelin09htl at gmail.com>
> To: Carol Torrence <ctorrence at kmcpa.com>
> Cc: Histonet <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Pregnancy guide for working in histology
> Message-ID:
> <CAOEzYbS2JN3DFqkjJFj+Tx7r+rVf3hFvbKLyXFQyWegsyfNwVw at mail.gmail.com>
> Content-Type: text/plain; charset="UTF-8"
>
> Hi Carol,
> I did a poster presentation on this topic at the NSH Symposium in Long
> Beach, 2016.
> I would be happy to send you a copy of my poster. I also wrote a blog
> article about my research for the poster found here:
> https://www.fixationonhistology.com/home/my-pregnancy-in-the-lab-researching-safety-considerations
> The Block also has the abstract, podcast interview, and a Safety Snippet of
> my poster.
> Please contact me directly if you have any other questions or I can help in
> any way.
> Katelin
>
>
> On Thu, Sep 6, 2018, 10:01 AM Carol Torrence via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
>
>> Good morning!
>>
>> Could some of you chime in on guidelines you go by for those employee that
>> are expecting a baby. I have removed employee from xylene exposure during
>> staining and cover slipping but am on the fence about grossing. At this
>> time the employee has been removed from grossing. All grossing is done
>> under an exhaust hood. Our exposure badges have always read well below
>> limits. Thanks in advance!
>>
>> Carol M. Torrence, HT(ASCP)
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet at lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
> ------------------------------
>
> Message: 6
> Date: Fri, 7 Sep 2018 06:06:32 +0000
> From: Histology <Histology at nwlabs.co.uk>
> To: P Sicurello <patpxs at gmail.com>
> Cc: "jhill at vet.k-state.edu" <jhill at vet.k-state.edu>, HistoNet
> <Histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Elephant Tissues
> Message-ID: <F48A2D1E-FED3-4D1B-B367-87597D74CCA0 at nwlabs.co.uk>
> Content-Type: text/plain; charset="utf-8"
>
> Hi Jennifer,
>
> If it is skin extending the fixing and processing time should cure any processing issues. If after this it?s still not working a short pre fix in khales fixative and surface softening before sectioning with 0.02% sodium hydroxide may help.
>
> Hope this helps!
>
> Stuart Beaver BSc(Hons)
> Head of Veterinary Histology/Cytology
> +447568543761
>
> On 5 Sep 2018, at 22:46, P Sicurello <patpxs at gmail.com<mailto:patpxs at gmail.com>> wrote:
>
> Jennifer,
>
> I have worked on mouse, rat, rabbit, sea lion, harbor seal, killer whale,
> giraffe, and even human mummy tissues. With the exception of the mummy
> tissue being a bit dry, they all embedded and cut like human tissue.
>
> What is it that is making them hard to cut?
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 200 Arbor Drive
>
> San Diego, CA 92103
>
> (P): 619-543-2872
>
>
>
> *Confidentiality Notice*: The information transmitted in this e-mail is
> intended only for the person or entity to which it is addressed and may
> contain confidential and/or privileged material. Any review,
> retransmission, dissemination or other use of or taking of any action in
> reliance upon this information by persons or entities other than the
> intended recipient is prohibited. If you received this e-mail in error,
> please contact the sender and delete the material from any computer.
>
>
> On Wed, Sep 5, 2018 at 10:38 AM Jennifer Phinney via Histonet <
> histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>> wrote:
>
> Hello Histonetters,
> Does anyone have experience processing and cutting elephant tissues? Any
> tips, tricks, or advice? My lab has had some elephant cases recently and
> the tissues are unexpectedly (to us) difficult to cut.
>
> Thanks for any help,
> Jennifer Phinney QIHC
> Kansas State University
> Veterinary Diagnostic Lab
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu<mailto:Histonet at lists.utsouthwestern.edu>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 7
> Date: Fri, 7 Sep 2018 09:56:00 +0200
> From: "Dr. Michael Gudo (Morphisto GmbH)" <michael.gudo at morphisto.de>
> To: Jennifer Phinney <jhill at vet.k-state.edu>
> Cc: P Sicurello <patpxs at gmail.com>, HistoNet
> <Histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Elephant Tissues
> Message-ID: <3F945ADB-2465-44A3-8342-EDCF98917267 at morphisto.de>
> Content-Type: text/plain; charset=utf-8
>
> Hello Jennifer,
>
> I do not have experiences with elephant tissues, but with other tissues that have thick connective fibres. Sodium hydroxide may help, however, I would recommend resin embedding, too. For this purpose Technovit 7100 would be a good and easy to use resin. Its easy to infiltrate and can be cut with rotary microtomes with some special knifes, and there are many stains that work on Technovit 7100 embedded specimens. Please let me know, if you need further information. We have a local dealer in US.
>
> Kind regards
> Michael
>
>> Am 06.09.2018 um 16:41 schrieb Jennifer Phinney via Histonet <histonet at lists.utsouthwestern.edu>:
>>
>> Hi Paula,
>> One of my pathologists thinks it could be the collagen in the tissues making them difficult to cut. We have tried nair, fabric softener, and even decaling the tissues and nothing helps. The tissues shred immediately when trying to section making it impossible in some cases to actually get a slide. When I melted a particularly difficult block down to separate out the different tissues, it turned out to be the lymph node that was the worst.
>>
>> We are going to try some of the cream you use to soften scar tissues to see if that has any effect.
>>
>> As a veterinary histology lab we routinely work on a variety of different species, and so far the elephant tissue is the only one giving us problems.
>>
>> Thanks for everyone?s help,
>> Jennifer
>>
>> From: P Sicurello <patpxs at gmail.com>
>> Sent: Wednesday, September 5, 2018 4:46 PM
>> To: Jennifer Phinney <jhill at vet.k-state.edu>
>> Cc: HistoNet <Histonet at lists.utsouthwestern.edu>
>> Subject: Re: [Histonet] Elephant Tissues
>>
>> Jennifer,
>>
>> I have worked on mouse, rat, rabbit, sea lion, harbor seal, killer whale, giraffe, and even human mummy tissues. With the exception of the mummy tissue being a bit dry, they all embedded and cut like human tissue.
>>
>> What is it that is making them hard to cut?
>>
>> Sincerely,
>>
>> Paula Sicurello, HTL (ASCP)CM
>>
>> Histotechnology Specialist
>>
>> UC San Diego Health
>>
>> 200 Arbor Drive
>>
>> San Diego, CA 92103
>>
>> (P): 619-543-2872
>>
>>
>>
>> Confidentiality Notice: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer.
>>
>>
>> On Wed, Sep 5, 2018 at 10:38 AM Jennifer Phinney via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>> wrote:
>> Hello Histonetters,
>> Does anyone have experience processing and cutting elephant tissues? Any tips, tricks, or advice? My lab has had some elephant cases recently and the tissues are unexpectedly (to us) difficult to cut.
>>
>> Thanks for any help,
>> Jennifer Phinney QIHC
>> Kansas State University
>> Veterinary Diagnostic Lab
>> _______________________________________________
>> Histonet mailing list
>> Histonet at lists.utsouthwestern.edu<mailto:Histonet at lists.utsouthwestern.edu>
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> _______________________________________________
>> Histonet mailing list
>> Histonet at lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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