[Histonet] Unstained slides

[P Sicurello]

How about frozen sections cut for immunofluorescence stored at -20?

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



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On Wed, Sep 5, 2018 at 1:12 PM Hobbs, Carl via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

>
> Hi
>
> Depends on what you mean by cryosections.
> Unfixed/fixed?
> Stored at RT, 4C, -20C, -80C.
> Stored dry or in glycerol
>
> So many variables!
> My opinion is to store blocks and cut sections as required.
> Least variables.
> Sure, one loses some tissue everytime one cuts anew....a good thing.
>
> It IS complicated so, a project has to be thought out well in advance.
>
> Stimulating post
>
>
>
> Carl Hobbs FIBMS
> Histology and Imaging Manager
> Wolfson CARD
> Guys Campus, London Bridge
> Kings College London
> London
> SE1 1UL
>
> 020 7848 6813
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