[Histonet] Frozen sections and cold acetone

[Hobbs, Carl]

I have always "fixed" in RT acetone for 10 mins
Have compared 0-60 mins/4C to RT acetone
Lower temps just limit the rate of reaction, imho.
I note "nuclear streaming" when I use acetone at any temp/time.

Imho, acetone is not an effective  fixative....it's a delipidiser.
So, give it 10 mins at RT.
If anyone thinks it's effective because it is a dehydrant….well, one re- hydrates the section to carry out IHC/ICC/IF.

It works very well for only a few abs.

When testing new abs out on FS/cell monolayers ( unfixed) I always compare Formalin, Acetone, Methanol and methanol/acetone 1:1
Imho

Best wishes to a great site/membership.
  
 
Carl