[Histonet] Frozen sections and cold acetone...

[Bryan Llewellyn]

Cold acetone, and cold ethanol, were used to fix tissues because they 
left enzymes unaffected and still demonstrable. This was in the early 
days of enzyme histochemistry. Pearse' Histochemistry: Theoretical and 
applied,3rd edition, volume 1, page 85 discusses it. I could send a scan 
if you wanted.

Bryan Llewellyn



Morken, Timothy via Histonet wrote:
> Can anyone give me a rational for using cold (refrig or freezer-temp) acetone to fix frozen sections? Or a rational for using RT acetone.
>
> This is for kidney or muscle bx frozens for immmunofluroescence or immunoperoxidase staining.
>
> Normally they air dry for at least 15 minutes (just waiting for frozen sectioning to be completed) before going into acetone. Just wondering if we can reduce complexity...
>
> I haven't seen anything saying why cold acetone is used, just instructions to do so. I always wonder about such things...
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>