[Histonet] Leica Bond Max artifact
Dr. Jason Rosen
jrosen at totalderms.com
Thu Oct 11 12:36:12 CDT 2018
Hi Greg,
It has been a problem for months across hundreds of kits. We added some washes and more dips in the DI water after the run to the protocol. It helps a little bit. I am going to try a more things in the next few weeks and I will keep the community updated.
-----Original Message-----
From: Greg Dobbin <greg.dobbin at gmail.com>
Sent: Thursday, October 11, 2018 11:04 AM
To: Linda.Margraf at cookchildrens.org
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Leica Bond Max artifact
Hi Linda,
Is this a problem that has just started or has the red kit always had this artefact?
- If it just started, perhaps it is a problem with a particular kit lot
number (or numbers). If so, get Leica Tech service involved to see if
anyone else has reported a problem.
- If it has been an ongoing issue for the red kit then perhaps you need
to modify the Red Kit protocol by adding additional (and/or longer) wash
steps. Here again Tech Service can help you with this.
- The only other thing I can think to ask is: Do you have anyone new
operating the instrument? Could a new person be putting undiluted Bond Wash
in the bulk container?
Let us know what you find out. Good luck.
Greg
--
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE C0A 1P0
*Everything in moderation...even moderation itself**!*
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