[Histonet] Leica Bond Max artifact

[Dr. Jason Rosen]

Hi Greg, 

It has been a problem for months across hundreds of kits. We added some washes and more dips in the DI water after the run to the protocol. It helps a little bit. I am going to try a more things in the next few weeks and I will keep the community updated. 

-----Original Message-----
From: Greg Dobbin <greg.dobbin at gmail.com> 
Sent: Thursday, October 11, 2018 11:04 AM
To: Linda.Margraf at cookchildrens.org
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Leica Bond Max artifact

Hi Linda,
Is this a problem that has just started or has the red kit always had this artefact?

   - If it just started, perhaps it is a problem with a particular kit lot
   number (or numbers). If so, get Leica Tech service involved to see if
   anyone else has reported a problem.


   - If it has been an ongoing issue for the red kit then perhaps you need
   to modify the Red Kit protocol by adding additional (and/or longer) wash
   steps. Here again Tech Service can help you with this.
   - The only other thing I can think to ask is: Do you have anyone new
   operating the instrument? Could a new person be putting undiluted Bond Wash
   in the bulk container?

Let us know what you find out. Good luck.
Greg

--
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE      C0A 1P0


*Everything in moderation...even moderation itself**!*