[Histonet] H&E for H. pylori?

Tue Oct 9 17:59:15 CDT 2018

The following might be useful:

Tazawa, K., & Tsutsumi, Y. (1998). Effect of prolonged staining with hematoxylin on detecting Helicobacter pyiori in hematoxylin‐eosin‐stained gastric mucosa. Pathology international, 48(6), 448-452.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, 10 October 2018 6:46 AM
To: P Sicurello; HistoNet
Subject: Re: [Histonet] H&E for H. pylori?

Paula:"Optimizing" H&E to detect H. piloris is a rout you should not attempt because H&E cannot be "optimized" for that.Giemsa is one way or, even better, modified Steiner stain. The only disadvantage modified Steiner has is using radioactive uranium nitrate, but I developed a procedure where 0.02% aq. phosphotungstic acid solution can be used instead. IHC will be way more expensive.
René 

    On Tuesday, October 9, 2018 2:03 PM, P Sicurello via Histonet <histonet at lists.utsouthwestern.edu> wrote:

 Good Morning Listers,

Has anyone out there optimized an H&E for H. pylori?

Sure we can run a giemsa or and IHC but what fun is that?

Send me your ideas and make the day of our GI pathologists.

Thanks oodles.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

9300 Campus Point Drive

La Jolla, CA 92037
(P): 858-249-5610

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