[Histonet] AFB STAIN

[WILLIAM DESALVO]

I completely agree with Tony. Not likely to see any sloughing off (shedding) of organisms from FFPE tissue blocks.

William DeSalvo

________________________________
From: Tony Henwood (SCHN) via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Thursday, October 4, 2018 1:54 PM
To: adesupo2002 at hotmail.com; Laurie Colbert
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] AFB STAIN

Cross-contamination has not been proven in FFPE tissues.

I have not seen it in nearly 40 years of practice

Do you have any evidence of cross-contamination in FFPE?

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

________________________________________
From: Laurie Colbert via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Thursday, October 4, 2018 10:36 PM
To: adesupo2002 at hotmail.com; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] AFB STAIN

You should put the AFB control on a separate slide to prevent cross-contamination.

Laurie Redmond


-----Original Message-----
From: ADESUPO ADESUYI via Histonet <histonet at lists.utsouthwestern.edu>
To: histonet <histonet at lists.utsouthwestern.edu>
Sent: Wed, Oct 3, 2018 7:58 pm
Subject: [Histonet] AFB STAIN

Hello,
I have a question please. For the AFB Stain, do we put both the control tissue and the patient on the same slide?

Thanks,
Ade
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