[Histonet] PAS Stain

[Mayer,Toysha N]

Laurie,

The warm water helps the color of the Schiff develop in a more vibrant pattern.  If you allow the slides to sit in warm water for 2 min, then rinse you also do not have to use the metabisulfite rinses to remove excess stain.

Toysha N. Mayer D.H.Sc., MBA, HT(ASCP)
Assistant Professor/Education Coordinator
HTL Program
MD Anderson School of Health Professions
713.563.3481
tnmayer at mdanderson.org









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Message: 3
Date: Mon, 5 Nov 2018 16:44:23 +0000 (UTC)
From: Laurie Redmond <boznpl at aol.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] PAS Stain
Message-ID: <1715295197.1084926.1541436263630 at mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

Does anyone else out there use warm water to rinse the PAS slides after the Schiff's reagent?? Can anyone comment on whether using warm water vs. cold water would have any affect on the stain?
Laurie Redmond HT (ASCP)

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End of Histonet Digest, Vol 180, Issue 4
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