[Histonet] Clear-Rite 3 dirty slides

[Allan Wang]

Thanks all, looks like the consensus is that water into the clearing agent
causes it and I should change out alcohol more frequently.

Allan

On Wed, Oct 31, 2018 at 5:46 PM Allan Wang <allanvv at gmail.com> wrote:

> Hi histonet,
>
> For coverslipping Ventana IHC slides I have dish soap, rinse, alcohol 80%,
> 100%, 100%, 100%, followed by three Clear-Rite 3 instead of xylene. In the
> past I didn't have this issue, but now I frequently get a lot of residue on
> the slides after they enter the Clear-Rite.
>
> If I completely dump all 3 containers of Clear-Rite 3 it gets rid of the
> problem but I'd have to do this every month which is wasteful. If I just
> dump the first container and move everything up, the problem still remains.
>
> Images:
> https://i.imgur.com/GTQJe2W.jpg
> https://i.imgur.com/np8B5P2.jpg
> https://i.imgur.com/SLypUAy.jpg
>
> Anyone know what this contamination is and the best way to reduce it?
>
> Allan
>