[Histonet] Umbilical Cord Embedding

[Michael Gudo (Morphisto GmbH)]

Dear Jessica,

we have done embedding in paraffine and the results are quite good. The critical point is fixation. Due to the high proportion of water in the tissue, the best fixation is possible with Bouin-solution or - if you want to do IHC stainings, Zamboni-fixation (which is a variant of Bouin with a buffered pH value).

With trichromatic or polychromatic stainings you will get very colorful results.

Best regards
Michael


> Am 24.05.2018 um 21:01 schrieb Jessica Riggleman via Histonet <histonet at lists.utsouthwestern.edu>:
> 
> Hello,
> Does anyone have any experience embedding umbilical cord tissue? Preferably paraffin but we may try plastic as well. Any help/protocols/etc. would be appreciated.
> 
> Thank You,
> Jessica
> 
> 
> _____________________________________________________________________
> 
> Jessica Riggleman | Research Associate
> 
> Globus Medical, Inc.
> Valley Forge Business Center
> 2560 General Armistead Avenue | Audubon, PA 19403
> Ph: (610) 930-1800 ext. 2583 | Fax:
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MORPHISTO GmbH
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