[Histonet] Histonet Digest, Vol 174, Issue 7- Benchtop fume hood. (Alan Wagg)

Thu May 10 17:59:44 CDT 2018

Hey Alan,

I use a Airfiltronix Ductless Bench Top Hood and it meets all my needs.

I gross about 7500-8000 GI biopsies per year and do any hand cover slipping under this hood (which is rare if I can help it).

The unit is made of clear acrylic, is see thru and is framed out in light weight aluminum. It has a clear Velcroed thick mil roll up sash which is very easy to work with. The system has two blower units on top. Both units have 2 inch carbon filters that I purchase from Mercedes Medical and replace twice a year.

I did add a grated plastic secondary containment box which I bought through Grainger  to place the unit on so that I could keep my formalin cube under the hood and not worry about spillage. This also elevated the work surface off the counter by 8-10 inches so you are not leaning over the work and therefore saving your back.

I had this unit tested by an outside agency along with the whole lab before we started operation and then again after we started operation. Our PEL numbers for Formalin, Alcohol and Xylene were barley negligible. I have used the badge system every year since the original testing  with the same results. This to me is the key to selecting any hood, does it keep the PEL numbers within exposure limits.

The purchase price is around $2800.00 + filters @ $180 ea.

Hope this helps and good luck.

Brent Adams – BS, LPN, HT

www.acadianagastro.com

Acadiana Gastroenterology Associates, LLC
439 Heymann Blvd
Lafayette, LA  70503

tel:  (337) 269-1126
fax:  (337) 269-1476

________________________________
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Sent: Thursday, May 10, 2018 12:00 PM
To: histonet at lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 174, Issue 7

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Today's Topics:

   1. Re: Paraffin infiltration issue, will this impact IHC?
      (Greg Dobbin)
   2. Histotech Position - Chandler AZ (Office Manager)
   3. IHC and Histogel (Paula)
   4. Re: IHC and Histogel (dusko trajkovic)
   5. Re: IHC and Histogel (John Shelley)
   6. Shandon Veristain Gemini Autostainer (Charles Riley)
   7. benchtop fume hoods (Histology)
   8. Re: Shandon Veristain Gemini Autostainer (Mike Toole)
   9. Re: benchtop fume hoods (Allan Wang)

----------------------------------------------------------------------

Message: 1
Date: Wed, 9 May 2018 15:26:57 -0300
From: Greg Dobbin <greg.dobbin at gmail.com>
To: modz9636 at gmail.com, histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Paraffin infiltration issue, will this impact
        IHC?
Message-ID:
        <CAHG6iQyJyH7HH2x8gibxkaA8BBEjn_=GSYhKvQ2r5nEL=qoS1Q at mail.gmail.com>
Content-Type: text/plain; charset="UTF-8"

Hi Merissa,
As far as I am aware, insufficient paraffin infiltration would only affect
sectioning. The epitopes that we we are attempting to stain with IHC are
affected by pre-analytic factors such as fixation, cold ischemic time and
perhaps heat (too much) but plus or minus wax should not be an issue if you
are able to obtain good sections. Have you stained these poorly infiltrated
specimens with H&E? Are you certain you have good sections with all tissue
components represented? That would be my main concern.
Greg

--
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE      C0A 1P0

*Everything in moderation...even moderation itself**!*

------------------------------

Message: 2
Date: Wed, 9 May 2018 14:26:22 -0700
From: Office Manager <jobposting2600 at gmail.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Histotech Position - Chandler AZ
Message-ID:
        <CAKRVzfqK4raB6+J=HnKDHFYQ2H=YOheHg-eVvnk7Du0KA=B9HA at mail.gmail.com>
Content-Type: text/plain; charset="UTF-8"

*Essential functions and responsibilities:*

   - Ensures compliance with all local, federal, CLIA, regulations.
   - Performs routine and non-routine activities involved in the
   preparation of slides for microscopic evaluation by pathologist(s),
   according to policies and procedures.
   - Responsible for embedding of surgical pathology specimens.
   - Responsible for the sectioning and microtomy of surgical pathology
   specimens.
   - Responsible for the routine staining, histochemical staining, and
   immunohistochemical staining of surgical pathology specimens for microscope
   evaluation and analysis.
   - Performs routine maintenance and cleaning of equipment and
   troubleshoots minor equipment failures. Documents remedial actions such as
   repairs or repeated tests.
   - Adheres to laboratory?s quality control policies and documents all
   quality control activities.
   - Maintains diagnostic viability of all specimens and ensures correct
   patient labeling.
   - Unpacks orders received; dates and stocks orders, in accordance with
   established policies and procedures.
   - Maintain a clean and safe prep work area in accordance with all
   laboratory and safety SOP?s.
   - Prepares and labels necessary stains and reagents in accordance with
   departmental procedures, policies and standards.
   - Reviews and performs QC on slides before taking the slides to the
   pathologist.
   - Maintenance of accurate work records and logs (i.e., Discrepancy Logs,
   Maintenance Logs, Quality Control Logs etc.)
   - Demonstrates a commitment to service, organizational values, and
   professionalism through appropriate conduct and demeanor at all times.
   - Works collaboratively and supports efforts of team members.
   - Protects patient information by adhering to professional standards
   including the Health Information Portability and Accountability Act (HIPAA).

*Knowledge and Skill:*

   - Must have 2+ years laboratory experience.
   - HT Certified preferred.
   - Must be precise when performing technical tasks.
   - Effective interpersonal skills, both in person and on the telephone.
   - High accuracy in work and attention to detail.

*Physical demands:*

   - Must have manual dexterity and motor coordination.
   - May involve sitting, standing, walking for long periods of time and/
   or sitting at the microtome for long periods of time.
   - Exposure to chemicals and fumes.

*Benefit Package:*

   - Health, Dental and Vision Insurance, 401K, Paid time off, Sick time
   and Holiday Pay.

*Required education:*

   - Associate?s Degree or higher

*Required experience and certification:*

   - Laboratory: 2 years
   - HT Certification ASCP preferred.

East Valley Gastroenterology & Hepatology Associates is an Equal
Opportunity Employer. This company does not and will not discriminate in
employment and personnel practices on the basis of race, sex, age,
handicap, religion, national origin or any other basis prohibited by
applicable law. Hiring, transferring and promotion practices are performed
without regard to the above listed items.

Job Type: Full-time
Reports to: Laboratory Manager
Schedule: 40 Hours per Week
Salary: DOE
FLSA Status: Hourly; Non-Exempt

To apply please reply to jobposting2600 at gmail.com or fax resume to (480)
786-6996, Attn:Isaac Malagon

------------------------------

Message: 3
Date: Thu, 10 May 2018 07:13:10 -0700
From: "Paula" <plucas at biopath.org>
To: <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] IHC and Histogel
Message-ID: <00b401d3e869$0802d5d0$18088170$@biopath.org>
Content-Type: text/plain;       charset="us-ascii"

Hello good morning everyone,

Can someone reply who knows if Histogel causes any interference with IHC
staining?

Thank you in advance,

Paula

Bio-Path Med Group

Fountain Valley, CA

------------------------------

Message: 4
Date: Thu, 10 May 2018 14:19:55 +0000 (UTC)
From: dusko trajkovic <dunatrsd at sbcglobal.net>
To: Paula <plucas at biopath.org>,  "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] IHC and Histogel
Message-ID: <421325481.1995533.1525961995270 at mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

We have used Histogel for years on cell pellets, and have not come across any IHC issues.Dusko

    On Thursday, May 10, 2018 7:13 AM, Paula via Histonet <histonet at lists.utsouthwestern.edu> wrote:

 Hello good morning everyone,

Can someone reply who knows if Histogel causes any interference with IHC
staining?

Thank you in advance,

Paula

Bio-Path Med Group

Fountain Valley, CA

_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 5
Date: Thu, 10 May 2018 14:22:07 +0000
From: John Shelley <jshelley at sbpdiscovery.org>
To: dusko trajkovic <dunatrsd at sbcglobal.net>, Paula
        <plucas at biopath.org>
Cc: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] IHC and Histogel
Message-ID:
        <C54F513DA7DA7547B37103A4B74BDAE9544A7F07 at CARRERA.ln.burnham.org>
Content-Type: text/plain; charset="iso-8859-1"

I agree with Dusko,

I have used histogel the same way and have also used it for FISH and ISH without any issues.

Kind Regards!
?
John J Shelley
Histology Core Manager
Sanford Burnham Prebys Medical Discovery Institute at Lake Nona
6400 Sanger Road???????????????????????????????
Orlando, FL 32827???????????????????????????????????
Tel: (407) 745-2000 Ext.2517
Lab: (407) 745-2119
Fax: (407) 745-2001
****email:? jshelley at SBPdiscovery.org ??****Please note email change

-----Original Message-----
From: dusko trajkovic via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, May 10, 2018 10:20 AM
To: Paula; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC and Histogel

We have used Histogel for years on cell pellets, and have not come across any IHC issues.Dusko

    On Thursday, May 10, 2018 7:13 AM, Paula via Histonet <histonet at lists.utsouthwestern.edu> wrote:

 Hello good morning everyone,

Can someone reply who knows if Histogel causes any interference with IHC
staining?

Thank you in advance,

Paula

Bio-Path Med Group

Fountain Valley, CA

_______________________________________________
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------------------------------

Message: 6
Date: Thu, 10 May 2018 10:50:52 -0400
From: Charles Riley <criley at dpspa.com>
To: Histo List <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Shandon Veristain Gemini Autostainer
Message-ID:
        <CAAQhB10gNz2nerS016YGGmR8x41bVMmH7E0J87icJKMYoc3Mhg at mail.gmail.com>
Content-Type: text/plain; charset="UTF-8"

Has anyone had any issues with this tissue stainer dropping or crushing
racks?  We use it to run our gyn and non gyn pap/thin prep slides.
Recently it has been tipping over the slide racks in the middle of the
procedure and breaking the slides

--

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs

------------------------------

Message: 7
Date: Thu, 10 May 2018 15:25:24 +0000
From: Histology <histo at pathlab.us>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] benchtop fume hoods
Message-ID:
        <09CFA3F99D5B2B42B88CDFB2FC4CFD8206EB91B3 at vdc01.domain.local>
Content-Type: text/plain; charset="us-ascii"

Hi all,

Can anyone share what type of benchtop fume hoods they are using in the lab?  This would be for manual coverslipping and grossing.

Thanks in advance!

------------------------------

Message: 8
Date: Thu, 10 May 2018 10:43:41 -0500
From: Mike Toole <mtoole at dcol.net>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Shandon Veristain Gemini Autostainer
Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB358CFE1F483 at mail>
Content-Type: text/plain; charset="us-ascii"

Charles,

The issue is most likely the handle for the rack, The rack has a moveable handle. Make sure the handle is securely in the upright position. It has pins and holes that should line up and click into place. If it is even slightly at an angle or not securely snapped into an upright position it may bump into the side of the stain pots (and really cause a mess).

Mike

-----Original Message-----
From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, May 10, 2018 9:51 AM
To: Histo List
Subject: [EXTERNAL] [Histonet] Shandon Veristain Gemini Autostainer

Has anyone had any issues with this tissue stainer dropping or crushing racks?  We use it to run our gyn and non gyn pap/thin prep slides.
Recently it has been tipping over the slide racks in the middle of the procedure and breaking the slides

--

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 9
Date: Thu, 10 May 2018 12:38:28 -0400
From: Allan Wang <allanw at biomax.us>
Cc: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] benchtop fume hoods
Message-ID:
        <CA+3cvL5ecd_R_NfqXdr5oUyZXgpPyQ2C+GXOYz5aLKjrbM2fug at mail.gmail.com>
Content-Type: text/plain; charset="UTF-8"

I'd like to know as well.

Thanks,
Allan

On Thu, May 10, 2018 at 11:25 AM, Histology via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi all,
>
> Can anyone share what type of benchtop fume hoods they are using in the
> lab?  This would be for manual coverslipping and grossing.
>
> Thanks in advance!
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

------------------------------

Subject: Digest Footer

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------------------------------

End of Histonet Digest, Vol 174, Issue 7
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