[Histonet] Identification of bacteria on an acrylic lens prosthesis

[Terri Braud]

I'm certified in EM. My suggestion would be to use SEM as opposed to TEM. The scanning electron microscope (SEM) can also be useful to reveal morphological features of isolated organisms as well as for diagnosis.  There are numerous articles citing this technique. I believe the UC Berkley has an SEM in their lab.    I hope this helps.  Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

Today's Topics:
   1. Prosthetic lens processing, cutting? (Morken, Timothy)
Message: 1
Date: Thu, 8 Mar 2018 19:30:35 +0000
From: "Morken, Timothy" <Timothy.Morken at ucsf.edu>
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Prosthetic lens processing, cutting?


Hi all,
Does anyone have any experience with embedding an acrylic prosthetic lens for EM, or any other way ? We have a case where they wanted EM on a lens to identify a  specific bacteria. We have never done it and it would take some work to figure it out. Also, I told them that unless there is some morphologic marker for the bacteria, EM would not help much identifying it.
Any ideas?
Any labs that handle this kind of thing? They are willing to send it out.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center


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