[Histonet] New Bone Stain

[Jessica Riggleman]

Hey Jennifer,
Thanks for your help. I have two questions:

1. What is the point of decalcifying if they are in plastic?
2. Did you see the counterstain? Right now only the bone is staining. How long did you leave these in the counterstain for?
Thanks!
Jessica

From: Mac Donald, Jennifer [mailto:jmacdonald at mtsac.edu]
Sent: Tuesday, July 10, 2018 4:07 PM
To: Jessica Riggleman <jriggleman at globusmedical.com>; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] New Bone Stain

I used to do it on plastic sections of in-de-calcified bone biopsies. It’s a silver reaction.

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_____________________________________________________________________

Jessica Riggleman | Research Associate

Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:

Confidentiality Note:  This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof.


________________________________________
From: Jessica Riggleman <jriggleman at globusmedical.com>
Sent: Tuesday, July 10, 2018 1:01 PM
To: Mac Donald, Jennifer; histonet at lists.utsouthwestern.edu
Subject: RE: [Histonet] New Bone Stain

Thank You,
Have you ever tried this on thick histology sections? I have a titanium implant in this section, so thin is impossible.

Thank You,
Jessica

________________________________________

Jessica Riggleman | Research Associate

Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:
An ISO 13485 Registered Company







Confidentiality Note:  This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof.
From: Mac Donald, Jennifer [mailto:jmacdonald at mtsac.edu]
Sent: Monday, July 09, 2018 6:09 PM
To: Jessica Riggleman <jriggleman at globusmedical.com>; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] New Bone Stain

von Kossa will stain the mineralized bone black and a basic fuschin counter stain the osteoid will stain deep pink.

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From: Jessica Riggleman via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Monday, July 9, 2018 1:17 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] New Bone Stain

Hello,
I am trying to assess new bone vs. old bone in an animal model. Any suggestions on thick section stains? I have heard Van Gieson's?

Thank You,
Jessica


_____________________________________________________________________

Jessica Riggleman | Research Associate

Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:

Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof.


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