[Histonet] Mohs tissue

Wed Jan 10 12:11:38 CST 2018

We receive some of the mohs layers for permanents, but it is not usual.  The mohs tech just plops the specimen into the formalin container without trimming off  the OCT, and includes the inking diagram on the path slip.

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Subject: Histonet Digest, Vol 170, Issue 9

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Today's Topics:

   1. Mohs Tissue (Zimmerman, Billie)
   2. Excell Plus (Cheryl Crowder)
   3. cell blocks and frozen for IHC (Blanca Lopez)

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Message: 1
Date: Tue, 9 Jan 2018 18:08:00 +0000
From: "Zimmerman, Billie" <BZIMMERM at augusta.edu>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Mohs Tissue
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        <BN6PR03MB2594EDC9C0D31AE932334600CB100 at BN6PR03MB2594.namprd03.prod.outlook.com>

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How many of you are fixing and embedding residual tissue from Mohs surgery?

Billie Zimmerman MT(ASCP)QIHC

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Message: 2
Date: Tue, 9 Jan 2018 13:03:14 -0600
From: Cheryl Crowder <ccrowder25 at gmail.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Excell Plus
Message-ID:
        <CACb9vmbe3G=x8iisCqMmkDCfOMFs-YCsbTvz4iHhO_TTU6X4MA at mail.gmail.com>
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Hello - has anyone used the non-formalin fixative Excell Plus?  I have a
researcher who wants to use it. I'm particularly interested in it's
dehydration ability since it is mostly alcohol based.  Thank you.

Cheryl Crowder
LSU Ag Center

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Message: 3
Date: Wed, 10 Jan 2018 14:25:15 +0000
From: Blanca Lopez <Blanca.Lopez at UTSouthwestern.edu>
To: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] cell blocks and frozen for IHC
Message-ID: <0f79996326654bda963324a968a5e2d4 at SWMS13MAIL12.swmed.org>
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Hello! Histonettes,
Is anyone willing to guide me on how can I start preparing frozen sections and cell blocks to perform immunostains please. I never done this before. My mainly experience is being paraffin. What are the materials that you suggest like slides or specific reagents, fixation, pretreatments, etc. I currently use Dako Autostainer link 48 to do all my IHC.
Thank You:)

Blanca Lopez HT (ASCP)
UT Southwestern Medical Center
UTSTR Biorepository /Tissue Bank
Histology Lab
214-648-7598
blanca.lopez at utsouthwestern.edu<mailto:blanca.lopez at utsouthwestern.edu>

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