[Histonet] cell blocks and frozen for IHC

[Blanca Lopez]

Hello! Histonettes,
Is anyone willing to guide me on how can I start preparing frozen sections and cell blocks to perform immunostains please. I never done this before. My mainly experience is being paraffin. What are the materials that you suggest like slides or specific reagents, fixation, pretreatments, etc. I currently use Dako Autostainer link 48 to do all my IHC.
Thank You:)

Blanca Lopez HT (ASCP)
UT Southwestern Medical Center
UTSTR Biorepository /Tissue Bank
Histology Lab
214-648-7598
blanca.lopez at utsouthwestern.edu<mailto:blanca.lopez at utsouthwestern.edu>


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