[Histonet] Histonet Digest, Vol 171, Issue 15

Fri Feb 16 13:22:19 CST 2018

I use methyl acetate to de-plasticize my PMMA slides.. . 30 minutes, air
dry for a minute or two then re-hydrate in 100% and 95% alcohol, rinse in
water.  I make my own PMMA ,rather than using Technovit, maybe that has
something to do with it.  Has worked for me for 25+ years!

Diane

Diane Mahovlic, HT(ASCP)
Cleveland Clinic,
Cleveland, Ohio

On Fri, Feb 16, 2018 at 1:00 PM, <histonet-request at lists.utsouthwestern.edu>
wrote:

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> Today's Topics:
>
>    1. Re: Deplastifying MMA (Kusters, Ronald)
>    2. Re: [EXTERNAL] Deplastifying MMA (Dorothy Hu)
>    3. P16 Antibody (Jason McGough)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 16 Feb 2018 09:50:15 +0000
> From: "Kusters, Ronald" <ronald.kusters at pfmmedical.com>
> To: Reuel Cornelia <Reuel.Cornelia at tsrh.org>,
>         "histonet at lists.utsouthwestern.edu"
>         <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Deplastifying MMA
> Message-ID: <8e78115855214a058d4e6ccc137a2ed6 at EX-PFM-103-W.pfm-ag.net>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Dear Reuel,
> Maybe the Kulkzer Technovit manufacturers website provides you more
> information or you could contact their specialists directly through this
> link:
> http://kulzer-technik.de/en_kt/kt/maerkte/histologie/
> startseite_histologie.aspx
>
> Yours,
> Ronald
>
> Mit freundlichen Gr??en/Kind Regards
>
>
>  Ronald Kusters
> International Sales Manager Feather Brand Histotechnology
>
> pfm medical ag
> Wankelstra?e 60
> 50996 K?ln, Germany
> T +49 (2236) 9641 660
> F +49 (2236) 9641 99 660
> M +49 (171) 9144343
>
>
>
> -----Original Message-----
> From: Reuel Cornelia [mailto:Reuel.Cornelia at tsrh.org]
> Sent: maandag 12 februari 2018 22:07
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Deplastifying MMA
>
> Hello histonetters,
>
> I have a problem with my MMA plastic section on my slide. I could not
> remove the plastic MMA even if I leave the section slides in Xylene for
> several days with heat incubation at 60 degrees. This is the first time
> that happened to my section for several years of doing the same thing over.
> I was thinking that it was my Xylene lot number so I tried to change it but
> still does not remove the plastic, and then Itried different solvent
> Acetone, and Ethylene glycol Monoethyl ether but still it does not work.
> Can I ask for help if anyone knows what was going on and what would be the
> best way to remove this plastic from my section?
>
> Just to give you my embedding solution was MMA -94% , dibutly phthalate-
> 5% and perkadox 16- 0.5%. This have been my solution for years and I do not
> have any problem with the removal of plastic section until now. I was
> thinking that my MMA (M55909) different lot number from Sigma Aldrich may
> have cause this because even I tried to use the MMA to dissolve my plastic
> it does not work.
>
>
> Thank you for and any opinions or protocols are greatly appreciated.
>
>
> Reuel Cornelia
>
> TSRH
>
> 214-559-7766
>
> ----------------------------------------------------------------------
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> ------------------------------
>
> Message: 2
> Date: Fri, 16 Feb 2018 08:34:42 -0500
> From: Dorothy Hu <abtdhu at gmail.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] [EXTERNAL] Deplastifying MMA
> Message-ID:
>         <CAJ2-qz7tX+hYHAcApBc3=JtcWdxOoAcvor-dPeDjD8NPW=8z4Q@
> mail.gmail.com>
> Content-Type: text/plain; charset="UTF-8"
>
> Hi Lori,
>
> Would you please tell me which company and catalog # of  tetrahydrofuran
> you used before?
> For how many minutes you did for deplastifying in tetrahydrofuran?
> Thanks in advance.
>
> Dorothy
>
> Message: 3
> Date: Wed, 14 Feb 2018 20:05:16 +0000
> From: "Garcia, Lori, M.Sc." <lori.garcia at medtronic.com>
> To: "Reuel.Cornelia at tsrh.org" <Reuel.Cornelia at tsrh.org>
> Cc: "histonet at lists.utsouthwestern.edu"
>         <histonet at lists.utsouthwestern.edu>
> Subject:
> Message-ID:
>         <MWHPR05MB29764D1B79279A8FAA9A2F3EFDF50 at MWHPR05MB2976.
> namprd05.prod.outlook.com>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Reuel,
>
> One thing you could try is THF (tetrahydrofuran). We have used it in the
> past to rapidly dissolve the MMA on slides.
>
> Good luck,
> Lori
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 16 Feb 2018 09:31:11 -0700
> From: Jason McGough <jmcgough at clinlab.com>
> To: 'histonet at lists.utsouthwestern.edu'
>         <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] P16 Antibody
> Message-ID: <zarafa.5a87074f.7551.22b16a0c5eb8f3c0 at mail.clinlab.com>
> Content-Type: text/plain; charset=utf-8
>
> We are looking to bring in P16 to do in house but are having a hard time
> getting the stain to be clean. We are having issues with positive staining
> on tissue that should be negative. We are working with a concentrated
> antibody that we have diluted out to 1:6000 (which is 6 times the amount
> suggested from vendors 1:200-1:1000). We might need to change clones or
> vendors and were wondering if you could tell us what kind of p16 (clone)
> you use for staining and possible what vendor you get it from? Any
> information or suggestions you have would greatly be appreciated. Thank you.
>
>
> Jason McGough, HT(ASCP)
>
> Operations Manager
> Clinical Laboratory of the Black Hills
> 605-343-2267
> jmcgough at clinlab.com
> www.clinlab.com
>
>
>
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>
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> ------------------------------
>
> End of Histonet Digest, Vol 171, Issue 15
> *****************************************
>