[Histonet] Depalstify

[Reuel Cornelia]

Hello histonetters,
Regarding my first email. The thickness of my section is 5 micron.


Reuel

 I have a problem with my MMA plastic section on my slide. I could not remove the plastic MMA even if I leave the section slides in Xylene for several days with heat incubation at 60 degrees. This is the first time that happened to my section for several years of doing the same thing over. I was thinking that it was my Xylene lot number so I tried to change it but still does not remove the plastic, and then Itried different solvent Acetone, and Ethylene glycol Monoethyl ether but still it does not work. Can I ask for help if anyone knows what was going on and what would be the best way to remove this plastic from my section?

Just to give you my embedding solution was MMA -94% , dibutly phthalate- 5% and perkadox 16- 0.5%. This have been my solution for years and I do not have any problem with the removal of plastic section until now. I was thinking that my MMA (M55909) different lot number from Sigma Aldrich may have cause this because even I tried to use the MMA to dissolve my plastic it does not work.


Thank you for and any opinions or protocols are greatly appreciated.


Reuel Cornelia

TSRH

214-559-7766

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