[Histonet] Histonet Digest, Vol 171, Issue 4

[Tyrone Genade]

Hello all, and especially those that offered advice.

Thanks for the advice. I will be experimenting with the options today and
tomorrow.

Thanks for being so generous with information.

Kind regards

On Sun, Feb 4, 2018 at 12:00 PM, <histonet-request at lists.utsouthwestern.edu>
wrote:

> Date: Sat, 3 Feb 2018 13:50:11 -0500
> From: Bob Richmond <rsrichmond at gmail.com>
>
> Bryan Llewellyn refers you to
> http://stainsfile.info/StainsFile/stain/oversight/romanowsky.htm
>
> That method prescribes neutral buffered formalin fixation. Us old-timers
> preferred Zenker fixation, which you can't do today on account of the
> mercury in it. You may have to try different fixatives.
>
> The differentiation under microscopic control is really necessary - when I
> was a resident at Cornell Medical Center in NYC fifty years ago, the
> histotechs would push the residents off the double-headed microscopes when
> they sat down to differentiate the bone marrow biopsy section Giemsa
> stains.
>
> The old-timers used a 10% solution of colophonium rosin (almost pure
> abietic acid) in alcohol, instead of acetic acid, to differentiate the
> stain.
>
> You buy Giemsa or Wright or Romanovsky stain already dissolved in absolute
> alcohol. You have to be a fanatic about not contaminating your stock bottle
> with the tiniest trace of water.
>
> Bob Richmond
> Samurai Pathologist
> Maryville TN
>


-- 
Tyrone Genade
Orange City, Iowa
tel: (+1) 712 230 4101
http://tgenade.freeshell.org
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