[Histonet] Histonet Digest, Vol 177, Issue 16
John O'Brien
john at imebinc.com
Fri Aug 17 13:11:17 CDT 2018
Decal Cal Stat company was sold to Stat Lab Medical Products. Last year
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Today's Topics:
1. FW: Histotechnologist, nights, Saturday thru Thursday, UCSF
Medical Center, San Francisco, CA (Morken, Timothy)
2. Recall: Histotechnologist, nights, Saturday thru Thursday,
UCSF Medical Center, San Francisco, CA (Morken, Timothy)
3. Unstained slides - how long are they good for? (P Sicurello)
4. Re: Unstained slides - how long are they good for? (Jamie Watson)
5. Re: Unstained slides - how long are they good for?
(Tony Henwood (SCHN))
6. Decalcification for Bone Marrow Biopsies (Cartun, Richard)
7. Re: Unstained slides - how long are they good for?
(Morken, Timothy)
----------------------------------------------------------------------
Message: 1
Date: Thu, 16 Aug 2018 22:12:38 +0000
From: "Morken, Timothy" <Timothy.Morken at ucsf.edu>
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] FW: Histotechnologist, nights, Saturday thru
Thursday, UCSF Medical Center, San Francisco, CA
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A position is open for an night-shift histotech at UCSF, San Francisco.
Saturdays thru Thursdays. Starting time is flexible between 10:00 pm and
1:00 AM
The Histology Lab at UCSF is a full service lab with routine, special
stains, IHC and ISH. The workflow is fully barcoded and all equipment is
state of the art.
Pay ranges (hourly, depending on experience) with night shift differential
(to be confirmed by HR on offer):
Histotech 1:?? $41.07 to 51.16, $3.45
Histotech 2:? 44.09 to 54.91, $4.50
Histotech 3:? 48.16 to 60.07, $4.50
UCSF also offers extensive benefits:?
http://jobs.ucsfmedicalcenter.org/whychoose_employment.html
Apply at:
http://jobs.ucsfmedicalcenter.org/
Job ID is 16324
Contact Yuri Murphy, Histology Supervisor, ?at ?Yuri.murphy at ucsf.edu with
any questions.
Job Title
Histotechnologist - Pathology-Surgical/Histology (HISTO TCHNO 1, 2 OR 3) Job
ID 16324
Job Code 9065, 9066, 9067
Job Family, Technical & Technologist
Location: Mount Zion (SF)
Weekly Hours: 40 :? 100%
Appointment Type: Career
Department: Pathology-Surgical/Histology Shift" 8-hour Nights Full/Part
Time: Full-Time
Union Information: This classification is represented by a union
Favorite Job
At UCSF Health, our mission of innovative patient care, advanced technology
and pioneering research is redefining what's possible for the patients we
serve - a promise we share with the professionals who make up our team.?
Ranked by U.S. News & World Report as the number one hospital in California
- and among the top five in the country - UCSF Health is committed to
providing the most rewarding work experience while delivering the best care
available anywhere. In an environment that allows for continuous learning
and opportunities for professional growth, UCSF Health offers the ideal
atmosphere in which to best use your skills and talents.
Job Summary
Classification of Histotechnologist 1,?2, or 3 will be determined based upon
the qualifications of the selected candidate.
Under supervision (HT-I, II level) by Senior-level technologists, Lead
technologist and the Histology Supervisor, or direction (HT-III) by Lead
technologist and the Histology Supervisor, or direction (HT-Lead) by the
Histology Supervisor, the incumbent serves as a Histotechnologist in the
Histology laboratory.
Duties include tissue processing, embedding, paraffin sectioning, H&E
staining, Special Staining, specimen receipt and accessioning, Laboratory
information system operation, Quality Assurance record keeping, instrument
maintenance, intra-operative frozen sections, and other technical duties as
assigned, including coverage in the Immunohistochemistry laboratory and
Grossing lab as determined by the Lab Manager.
Rotates weekly between workstations within the lab. Work schedule is
variable to include Saturdays and holiday coverage as scheduled. Incumbent
must be able to flex work hours as needed to meet department operational
needs and cover work rotations.
Required Qualifications
. HT 1:
o Associate degree?or at least 60 semester hours of academic credit from a
regionally accredited college/university, with a combination of 12 semester
hours of biology and chemistry, AND one year of experience as a
histotechnologist, preferably in a high-volume hospital histology laboratory
within the last five years; or completion of an associate degree from an
accredited Histotechnology program o Excellent interpersonal and
communication skills . HT2:
o Current??HT license
o Associate degree or at least 60 semester hours of academic credit from a
regionally accredited college/university, with a combination of 12 semester
hours of biology and chemistry, AND two year of experience as a
histotechnologist, in a high-volume hospital histology laboratory within the
last five years;?or completion of an associate degree from an accredited
Histotechnology program o Demonstrated high volume and high quality
sectioning skills o Excellent interpersonal and communication skills . HT3:
o Current??HT license
o Associate degree or at least 60 semester hours of academic credit from a
regionally accredited college/university, with a combination of 12 semester
hours of biology and chemistry, AND four years of experience as a
histotechnologist in a comparable high-volume hospital histology laboratory
within the last five years o Demonstrated high-volume, high-quality
sectioning and staining skills o Ability to organize and prioritize
responsibilities and perform well under pressure to meet deadlines o
Excellent interpersonal and communication . The flexibility to orient and
work at all UCSF Medical Center locations
Preferred Qualifications
HT 1:
. One to two years?experience as a Histotechnologist . ASCP certification:
HT or HTL licensed
HT2:
. One to two years experience as a Histotechnologist . ASCP certified HTL
HT3:
. Five years experience as a Histotechnologist in a large hospital
laboratory . ASCP certified HTL
Licensure/Certification
HT 2 OR 3:
. Current?HT license
Living Pride Standards
Service Excellence
. Demonstrates service excellence by following the Everyday PRIDE Guide with
the UCSF Medical Center standards and expectations for communication and
behavior. These standards and expectations convey specific behavior
associated with the Medical Center's values: Professionalism, Respect,
Integrity, Diversity and Excellence, and provide guidance on how we
communicate with patients, visitors, faculty, staff, and students, virtually
everyone, every day and with every encounter. These standards include, but
are not limited to: personal appearance, acknowledging and greeting all
patients and families, introductions using AIDET, managing up, service
recovery, managing delays and expectations, phone standards, electronic
communication, team work, cultural sensitivity and competency. ??
. Uses effective communication skills with patients and staff; demonstrates
proper telephone techniques and etiquette; acts as an escort to any patient
or family member needing directions; shows sensitivity to differences of
culture; demonstrates a positive and supportive manner in which patients /
families/ colleagues perceive interactions as positive and supportive.
Exhibits team work skills to positively acknowledge and recognize other
colleagues, and uses personal experiences to model and teach Living PRIDE
standards.?
. Exhibits tact and professionalism in difficult situations according to
PRIDE Values and Practices . Demonstrates an understanding of and adheres to
privacy, confidentiality, and security policies and procedures related to
Protected Health Information (PHI) or other sensitive and personal
information.
. Demonstrates an understanding of and adheres to safety and infection
control policies and procedures.
. Assumes accountability for improving quality metrics associated with
department/unit and meeting organizational/departmental targets.?
Work Environment
. Keeps working areas neat, orderly and clutter-free, including the
hallways. Adheres to cleaning processes and puts things back where they
belong. Removes and reports broken equipment and furniture.
. Picks up and disposes of any litter found throughout entire facility.?
. Posts flyers and posters in designated areas only; does not post on walls,
doors or windows.
. Knows where the Environment of Care Manual is kept in department; corrects
or reports unsafe conditions to the appropriate departments. ?
. Protects the physical environment and equipment from damage and theft.
The flexibility to orient and work at all UCSF Medical Center locations is
required.
Equal Employment Opportunity
The University of California San Francisco is an Equal
Opportunity/Affirmative Action Employer. All qualified applicants will
receive consideration for employment without regard to race, color,
religion, sex, sexual orientation, gender identity, national origin, age,
protected veteran or disabled status, or genetic information. Further
information about the University of California, San Francisco, is available
at diversity.ucsf.edu. UCSF seeks candidates whose skills, and personal and
professional experience, have prepared them to contribute to our commitment
to diversity and excellence, and the communities we serve.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of
Pathology UC San Francisco Medical Center
------------------------------
Message: 2
Date: Thu, 16 Aug 2018 22:52:24 +0000
From: "Morken, Timothy" <Timothy.Morken at ucsf.edu>
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Recall: Histotechnologist, nights, Saturday thru
Thursday, UCSF Medical Center, San Francisco, CA
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Morken, Timothy would like to recall the message, "Histotechnologist,
nights, Saturday thru Thursday, UCSF Medical Center, San Francisco, CA".
------------------------------
Message: 3
Date: Thu, 16 Aug 2018 16:48:40 -0700
From: P Sicurello <patpxs at gmail.com>
To: HistoNet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Unstained slides - how long are they good for?
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Hello My Fellow Histologists,
Happy Friday Eve.
The question has come up...... How long are *unstained* slides good for?
Not for H&E but tests like IHC and molecular testing. These slides have
been cut, stored at room temperature, not sealed in anyway, and kept in a
cardboard box.
Please let me know what your opinions are and what your retention policy is
concerning *unstained* slides.
Thanks oodles.
Sincerely,
Paula Sicurello, HTL (ASCP)CM
Histotechnology Specialist
UC San Diego Health
200 Arbor Drive
San Diego, CA 92103
(P): 619-543-2872
*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material. Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited. If you received this e-mail in error,
please contact the sender and delete the material from any computer.
------------------------------
Message: 4
Date: Fri, 17 Aug 2018 01:12:24 +0000
From: Jamie Watson <jamie at watson-home.com>
To: P Sicurello <patpxs at gmail.com>, HistoNet
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Unstained slides - how long are they good for?
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It depends on the stability of the protein and fixation, some are stable
for a week some for years. We use 6 months as a standard.
Jamie
On August 16, 2018 4:59:34 PM P Sicurello via Histonet
<histonet at lists.utsouthwestern.edu> wrote:
> Hello My Fellow Histologists,
>
> Happy Friday Eve.
>
> The question has come up...... How long are *unstained* slides good for?
> Not for H&E but tests like IHC and molecular testing. These slides have
> been cut, stored at room temperature, not sealed in anyway, and kept in a
> cardboard box.
>
> Please let me know what your opinions are and what your retention policy
is
> concerning *unstained* slides.
>
> Thanks oodles.
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 200 Arbor Drive
>
> San Diego, CA 92103
>
> (P): 619-543-2872
>
>
>
> *Confidentiality Notice*: The information transmitted in this e-mail is
> intended only for the person or entity to which it is addressed and may
> contain confidential and/or privileged material. Any review,
> retransmission, dissemination or other use of or taking of any action in
> reliance upon this information by persons or entities other than the
> intended recipient is prohibited. If you received this e-mail in error,
> please contact the sender and delete the material from any computer.
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Fri, 17 Aug 2018 01:30:20 +0000
From: "Tony Henwood (SCHN)" <tony.henwood at health.nsw.gov.au>
To: P Sicurello <patpxs at gmail.com>
Cc: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Unstained slides - how long are they good for?
Message-ID:
<1c96c1bd524c4ffd9974606bde1aa3d8 at SVDCMBX-MEX024.nswhealth.net>
Content-Type: text/plain; charset="iso-8859-1"
Will definitely depend on the antibody you are using. Some references:
Jacobs, T. W., Prioleau, J. E., Stillman, I. E., & Schnitt, S. J. (1996).
Loss of tumor marker-immunostaining intensity on stored paraffin slides of
breast cancer. JNCI: Journal of the National Cancer Institute, 88(15),
1054-1059.
Manne, U., MYERS, R. B., SRIVASTAVA, S., & GRIZZLE, W. E. (1997). Re: loss
of tumor marker-immunostaining intensity on stored paraffin slides of breast
cancer. Journal of the National Cancer Institute, 89(8), 585-586.
Bertheau, P., Cazals-Hatem, D., Meignin, V., de Roquancourt, A., V?rola, O.,
Lesourd, A., ... & Janin, A. (1998). Variability of immunohistochemical
reactivity on stored paraffin slides. Journal of clinical pathology, 51(5),
370-374.
Olapade-Olaopa, E. O., Mackay, E. H., & Habib, F. K. (1998). Variability of
immunohistochemical reactivity on stored paraffin slides. Journal of
clinical pathology, 51(12), 943.
Wester, K., Wahlund, E., Sundstr?m, C., Ranefall, P., Bengtsson, E.,
Russell, P. J., ... & Busch, C. (2000). Paraffin section storage and
immunohistochemistry: effects of time, temperature, fixation, and retrieval
protocol with emphasis on p53 protein and MIB1 antigen. Applied
Immunohistochemistry & Molecular Morphology, 8(1), 61-70.
van den Broek, L. J., & van de Vijver, M. J. (2000). Assessment of problems
in diagnostic and research immunohistochemistry associated with epitope
instability in stored paraffin sections. Applied Immunohistochemistry &
Molecular Morphology, 8(4), 316-321.
Olapade-Olaopa, E. O., Ogunbiyi, J. O., MacKay, E. H., Muronda, C. A.,
Alonge, T. O., Danso, A. P., ... & Wong, A. J. (2001). Further
characterization of storage-related alterations in immunoreactivity of
archival tissue sections and its implications for collaborative multicenter
immunohistochemical studies. Applied Immunohistochemistry & Molecular
Morphology, 9(3), 261-266.
Mirlacher, M., Kasper, M., Storz, M., Knecht, Y., D?rm?ller, U., Simon, R.,
... & Sauter, G. (2004). Influence of slide aging on results of
translational research studies using immunohistochemistry. Modern pathology,
17(11), 1414.
DiVito, K. A., Charette, L. A., Rimm, D. L., & Camp, R. L. (2004). Long-term
preservation of antigenicity on tissue microarrays. Laboratory
investigation, 84(8), 1071.
Fergenbaum, J. H., Garcia-Closas, M., Hewitt, S. M., Lissowska, J., Sakoda,
L. C., & Sherman, M. E. (2004). Loss of antigenicity in stored sections of
breast cancer tissue microarrays. Cancer Epidemiology and Prevention
Biomarkers, 13(4), 667-672.
Hameed, O., & Humphrey, P. A. (2009). Immunohistochemical evaluation of
prostate needle biopsies using saved interval sections vs new recut sections
from the block: a prospective comparison. American journal of clinical
pathology, 131(5), 683-688.
Xie, R., Chung, J. Y., Ylaya, K., Williams, R. L., Guerrero, N., Nakatsuka,
N., ... & Hewitt, S. M. (2011). Factors influencing the degradation of
archival formalin-fixed paraffin-embedded tissue sections. Journal of
Histochemistry & Cytochemistry, 59(4), 356-365.
Seidu, M. A., Adams, A. R., Gyasi, R. K., Tettey, Y., Nkansah, D. O., &
Wiredu, E. K. (2013). Immunoreactivity of some epitopes in longtime
inappropriately stored paraffin-embedded tissues. Journal of
Histotechnology, 36(2), 59-64.
Nuovo, A. J., Garofalo, M., Mikhail, A., Nicol, A. F., Vianna-Andrade, C., &
Nuovo, G. J. (2013). The effect of aging of formalin-fixed paraffin-embedded
tissues on the in situ hybridization and immunohistochemistry signals in
cervical lesions. Diagnostic Molecular Pathology, 22(3), 164-173.
Grillo, F., Bruzzone, M., Pigozzi, S., Prosapio, S., Migliora, P., Fiocca,
R., & Mastracci, L. (2017). Immunohistochemistry on old archival paraffin
blocks: is there an expiry date?. Journal of Clinical Pathology,
jclinpath-2017.
Giunchi, F., Degiovanni, A., Daddi, N., Trisolini, R., Dell'Amore, A.,
Agostinelli, C., ... & Fiorentino, M. (2018). Fading With Time of PD-L1
Immunoreactivity in Non-Small Cells Lung Cancer Tissues: A Methodological
Study. Applied Immunohistochemistry & Molecular Morphology, 26(7), 489-494.
-----Original Message-----
From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Friday, 17 August 2018 9:49 AM
To: HistoNet
Subject: [Histonet] Unstained slides - how long are they good for?
Hello My Fellow Histologists,
Happy Friday Eve.
The question has come up...... How long are *unstained* slides good for?
Not for H&E but tests like IHC and molecular testing. These slides have
been cut, stored at room temperature, not sealed in anyway, and kept in a
cardboard box.
Please let me know what your opinions are and what your retention policy is
concerning *unstained* slides.
Thanks oodles.
Sincerely,
Paula Sicurello, HTL (ASCP)CM
Histotechnology Specialist
UC San Diego Health
200 Arbor Drive
San Diego, CA 92103
(P): 619-543-2872
*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material. Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
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please contact the sender and delete the material from any computer.
_______________________________________________
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Views expressed in this message are those of the individual sender, and are
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------------------------------
Message: 6
Date: Fri, 17 Aug 2018 14:19:36 +0000
From: "Cartun, Richard" <Richard.Cartun at hhchealth.org>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Decalcification for Bone Marrow Biopsies
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Help! For years we have used Decal-Stat for decalcifying our bone marrow
biopsies with good results. For the past month we have been having problems
with tissue loss and morphological damage with these specimens following
decalcification. Unfortunately, this was just brought to my attention.
Someone told me this morning that the company producing this product was
sold and the formulation may have changed. Is that true?
Richard
Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 972-1596
(860) 545-2204 Fax
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message, including any attachments.
------------------------------
Message: 7
Date: Fri, 17 Aug 2018 15:16:00 +0000
From: "Morken, Timothy" <Timothy.Morken at ucsf.edu>
To: P Sicurello <patpxs at gmail.com>
Cc: Histonet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Unstained slides - how long are they good for?
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Paula, since it is variable we strive to not have unstained slides. We had
kept them indefinitely, then when storage was overwhelming us we reduced it
to 2 months maximum. Now we require request for unstained to be ordered in
the system and delivered to the pathologist. We do not hold any in the lab.
We recut when new stains are ordered. In the past we had routinely cut
extras "just in case" but ended up with thousands of unstained slides that
were never used. Instead we trained everyone to reduce wastage and get good
sections from a cut block with minimal facing. We have not stored unstained
sections for many years and they do not seem to be missed.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-----Original Message-----
From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, August 16, 2018 4:49 PM
To: HistoNet
Subject: [Histonet] Unstained slides - how long are they good for?
Hello My Fellow Histologists,
Happy Friday Eve.
The question has come up...... How long are *unstained* slides good for?
Not for H&E but tests like IHC and molecular testing. These slides have
been cut, stored at room temperature, not sealed in anyway, and kept in a
cardboard box.
Please let me know what your opinions are and what your retention policy is
concerning *unstained* slides.
Thanks oodles.
Sincerely,
Paula Sicurello, HTL (ASCP)CM
Histotechnology Specialist
UC San Diego Health
200 Arbor Drive
San Diego, CA 92103
(P): 619-543-2872
*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material. Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon this information by persons or entities other than the
intended recipient is prohibited. If you received this e-mail in error,
please contact the sender and delete the material from any computer.
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Subject: Digest Footer
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