[Histonet] Unstained slides - how long are they good for?

Tony Henwood (SCHN) tony.henwood at health.nsw.gov.au
Thu Aug 16 20:30:20 CDT 2018

Will definitely depend on the antibody you are using. Some references:

Jacobs, T. W., Prioleau, J. E., Stillman, I. E., & Schnitt, S. J. (1996). Loss of tumor marker-immunostaining intensity on stored paraffin slides of breast cancer. JNCI: Journal of the National Cancer Institute, 88(15), 1054-1059.

Manne, U., MYERS, R. B., SRIVASTAVA, S., & GRIZZLE, W. E. (1997). Re: loss of tumor marker-immunostaining intensity on stored paraffin slides of breast cancer. Journal of the National Cancer Institute, 89(8), 585-586.

Bertheau, P., Cazals-Hatem, D., Meignin, V., de Roquancourt, A., Vérola, O., Lesourd, A., ... & Janin, A. (1998). Variability of immunohistochemical reactivity on stored paraffin slides. Journal of clinical pathology, 51(5), 370-374.

Olapade-Olaopa, E. O., Mackay, E. H., & Habib, F. K. (1998). Variability of immunohistochemical reactivity on stored paraffin slides. Journal of clinical pathology, 51(12), 943.

Wester, K., Wahlund, E., Sundström, C., Ranefall, P., Bengtsson, E., Russell, P. J., ... & Busch, C. (2000). Paraffin section storage and immunohistochemistry: effects of time, temperature, fixation, and retrieval protocol with emphasis on p53 protein and MIB1 antigen. Applied Immunohistochemistry & Molecular Morphology, 8(1), 61-70.

van den Broek, L. J., & van de Vijver, M. J. (2000). Assessment of problems in diagnostic and research immunohistochemistry associated with epitope instability in stored paraffin sections. Applied Immunohistochemistry & Molecular Morphology, 8(4), 316-321. 

Olapade-Olaopa, E. O., Ogunbiyi, J. O., MacKay, E. H., Muronda, C. A., Alonge, T. O., Danso, A. P., ... & Wong, A. J. (2001). Further characterization of storage-related alterations in immunoreactivity of archival tissue sections and its implications for collaborative multicenter immunohistochemical studies. Applied Immunohistochemistry & Molecular Morphology, 9(3), 261-266.

Mirlacher, M., Kasper, M., Storz, M., Knecht, Y., Dürmüller, U., Simon, R., ... & Sauter, G. (2004). Influence of slide aging on results of translational research studies using immunohistochemistry. Modern pathology, 17(11), 1414.

DiVito, K. A., Charette, L. A., Rimm, D. L., & Camp, R. L. (2004). Long-term preservation of antigenicity on tissue microarrays. Laboratory investigation, 84(8), 1071.

Fergenbaum, J. H., Garcia-Closas, M., Hewitt, S. M., Lissowska, J., Sakoda, L. C., & Sherman, M. E. (2004). Loss of antigenicity in stored sections of breast cancer tissue microarrays. Cancer Epidemiology and Prevention Biomarkers, 13(4), 667-672.

Hameed, O., & Humphrey, P. A. (2009). Immunohistochemical evaluation of prostate needle biopsies using saved interval sections vs new recut sections from the block: a prospective comparison. American journal of clinical pathology, 131(5), 683-688.

Xie, R., Chung, J. Y., Ylaya, K., Williams, R. L., Guerrero, N., Nakatsuka, N., ... & Hewitt, S. M. (2011). Factors influencing the degradation of archival formalin-fixed paraffin-embedded tissue sections. Journal of Histochemistry & Cytochemistry, 59(4), 356-365.

Seidu, M. A., Adams, A. R., Gyasi, R. K., Tettey, Y., Nkansah, D. O., & Wiredu, E. K. (2013). Immunoreactivity of some epitopes in longtime inappropriately stored paraffin-embedded tissues. Journal of Histotechnology, 36(2), 59-64.

Nuovo, A. J., Garofalo, M., Mikhail, A., Nicol, A. F., Vianna-Andrade, C., & Nuovo, G. J. (2013). The effect of aging of formalin-fixed paraffin-embedded tissues on the in situ hybridization and immunohistochemistry signals in cervical lesions. Diagnostic Molecular Pathology, 22(3), 164-173.

Grillo, F., Bruzzone, M., Pigozzi, S., Prosapio, S., Migliora, P., Fiocca, R., & Mastracci, L. (2017). Immunohistochemistry on old archival paraffin blocks: is there an expiry date?. Journal of Clinical Pathology, jclinpath-2017.

Giunchi, F., Degiovanni, A., Daddi, N., Trisolini, R., Dell'Amore, A., Agostinelli, C., ... & Fiorentino, M. (2018). Fading With Time of PD-L1 Immunoreactivity in Non-Small Cells Lung Cancer Tissues: A Methodological Study. Applied Immunohistochemistry & Molecular Morphology, 26(7), 489-494.

-----Original Message-----
From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Friday, 17 August 2018 9:49 AM
To: HistoNet
Subject: [Histonet] Unstained slides - how long are they good for?

Hello My Fellow Histologists,

Happy Friday Eve.

The question has come up......  How long are *unstained* slides good for?
Not for H&E but tests like IHC and molecular testing.  These slides have been cut, stored at room temperature, not sealed in anyway, and kept in a cardboard box.

Please let me know what your opinions are and what your retention policy is concerning *unstained* slides.

Thanks oodles.


Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872

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