[Histonet] Histonet Digest, Vol 177, Issue 9

Solis, Bryan BSolis at Fibrogen.com
Fri Aug 10 12:23:40 CDT 2018


We are looking into purchasing  Tissue-tek VIP 6 AI system. Is  anyone out there currently using this?  Can anyone provide some input/feedback on this? 


Bryan S.

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Sent: Friday, August 10, 2018 10:00 AM
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Subject: Histonet Digest, Vol 177, Issue 9

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Today's Topics:

   1. Iron counterstains (Cassie P. Davis)
   2. ADH 5 (Cassie P. Davis)


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Message: 1
Date: Fri, 10 Aug 2018 10:59:00 +0000
From: "Cassie P. Davis" <CDavis at che-east.org>
To: histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Iron counterstains
Message-ID: <ee3de7bc947741b9852bd256bc911b9b at che-east.org>
Content-Type: text/plain; charset="iso-8859-1"

I enjoy this forum so much, I get to use brain cells I haven't used in years!
When I saw your question about other counterstains for Iron Stain I kept thinking quick dip in Eosin (from you H&E) because I thought I remember having to do that in the 90's when we ran our of NFR. Histologist can be so resourceful with the right support. If I remember correctly light Eosin can also be used as a counterstain for GMS,  some have used a very pale Hematoxylin but I personally don't care for it because it wasn't enough contrst for me. Tony, thanks for speaking up, it's nice to know I haven't forgot eveything with all the automation.

Cassie Davis

Message: 6
Date: Wed, 8 Aug 2018 23:29:27 +0000
From: "Tony Henwood (SCHN)" <tony.henwood at health.nsw.gov.au>
To: "Campbell, Tasha M." <tmcampbe at fmh.org>
Cc: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Iron Stain
Message-ID:
        <4125cb6319d24437bbd26ff12d106dc8 at SVDCMBX-MEX024.nswhealth.net>
Content-Type: text/plain; charset="us-ascii"

Try your eosin stain used in your H&E or 1% Neutral red in 2% acetic acid

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA







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Message: 2
Date: Fri, 10 Aug 2018 11:51:12 +0000
From: "Cassie P. Davis" <CDavis at che-east.org>
To: histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] ADH 5
Message-ID: <75a06eb487494e5ba5b30959dbd9c13e at che-east.org>
Content-Type: text/plain; charset="iso-8859-1"


Hi Histoland folks,

        Are/were any of you fine folks using the ADH5 from Biocare on the Ventana instrument?

What are you doing now that the have replaced the panel with different antibodies which I already get from Ventana? My doctors really liked the panel, I have a couple of  ideas but I wanted to know what other labs are doing so I can offer the doctors more than two options.


Cassandra Davis
Histology Technician
AP Laboratory
302-575-8095
Email:  CDavis at che-east.org<mailto:CDavis at che-east.org>



Confidentiality Notice:
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