[Histonet] Iron counterstains

Cassie P. Davis CDavis at che-east.org
Fri Aug 10 05:59:00 CDT 2018

I enjoy this forum so much, I get to use brain cells I haven't used in years!
When I saw your question about other counterstains for Iron Stain I kept thinking quick dip in Eosin (from you H&E) because I thought I remember having to do that in the 90's when we ran our of NFR. Histologist can be so resourceful with the right support. If I remember correctly light Eosin can also be used as a counterstain for GMS,  some have used a very pale Hematoxylin but I personally don't care for it because it wasn't enough contrst for me. Tony, thanks for speaking up, it's nice to know I haven't forgot eveything with all the automation.

Cassie Davis

Message: 6
Date: Wed, 8 Aug 2018 23:29:27 +0000
From: "Tony Henwood (SCHN)" <tony.henwood at health.nsw.gov.au>
To: "Campbell, Tasha M." <tmcampbe at fmh.org>
Cc: "histonet at lists.utsouthwestern.edu"
        <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Iron Stain
        <4125cb6319d24437bbd26ff12d106dc8 at SVDCMBX-MEX024.nswhealth.net>
Content-Type: text/plain; charset="us-ascii"

Try your eosin stain used in your H&E or 1% Neutral red in 2% acetic acid

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

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