[Histonet] feathers

[Lauren Sweeney]

Hello Histoland,


A researcher brought us a group of poultry feather shafts to embed and section for routine and IHC staining. Has anyone out there had any experience with this that could share some advice on getting some successful sections?


So far we have tried facing the blocks and then applying "Nair for Men" for ten minutes to the block surface, followed by soaking the blocks for some time before attempting sectioning but still we have not had any luck. The sections turn out with large holes and blow apart on the water bath.


Any tips would be appreciated, thanks!


Lauren