[Histonet] Protocols for cryostat trimming and sectioning

[P Sicurello]

Hi Bernard,

If memory serves me correctly, a cryostat is like a microtome and does not
need validation - CAP requires a function check (look in the All Common
Checklist) and verification from the vendor that it was installed correctly
and it operates as it should.  Does it turn on?  Does it get cold?  Does it
cut?  Things like that.

I hope that helps.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



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On Mon, Sep 11, 2017 at 1:24 AM, Bernard Gregory Amparo via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Good day Hisonetters,
>
>
>
> I would like to ask about your protocol for the cryostats you are using in
> your lab. We are using a Leica CM1950 and we are now validating the machine
> for use.
>
> So could you please help me to gain some insights regarding this matter?
>
>
>
>
>
> Thanks!
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