[Histonet] validation inquiry regarding negative tissue

[Eddie Martin]

Hi Nancy,

The use of negative internal control tissue on your antibody testing for negative cases would have to be determined by your medical director/chief pathologist as acceptable. 

You may also use Lipoma tissue as a negative control tissue. It works as a negative control for just about every antibody I can think about. There may also be a few antibodies that would have false positive staining with a lipoma, but a pathologist could read through that stuff. 

Hope this helps!

Kind regards,
Eddie M.

Edward M. Martin, HTL, HT(ASCP), QIHC(ASCP)
Lead Hematopathology Medical Technologist
The National Institutes of Health
and
IHC Histotechnologist III
The Joint Pathology Center
Walter Reed NMMC
Bethesda, MD


> On Oct 24, 2017, at 1:16 PM, Nancy Schmitt <Nancy_Schmitt at pa-ucl.com> wrote:
> 
> Good Day!
> When performing validation on IHC, we use 20 positives and 20 negative cases.
> 
> When testing for negative, can the normal tissue surrounding the tumor be counted as negative?
> 
> Does the negative tissue need to be tumor that is negative for the AB or just normal tissue?
> 
> Thank you for your consideration,
> 
> Nancy
> Dubuque, IA
> Ph. 563-589-9810
> 
> 
> 
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