[Histonet] H&E stain protocols

Mayer,Toysha N TNMayer at mdanderson.org
Thu Oct 19 16:46:04 CDT 2017

We have a few questions to ask:
In your H&E protocols, what is your set up after eosin: 70, 95, 100, 100, xylene  x 3or 95, 100, 100, 100, xylene x 3, or another variation?
What brand of hematoxylin do you use? Eosin?
Do you filter daily or at all? Eosin?
How often do you change the hematoxylin? Eosin?
Do you start with manufacturer suggestions for filtering and changing? Protocols? 
Carson says one thing, Bancroft something else, and the manufacturer different. 
I was taught 80, eosin, 70, 95, 100, 100, xylene x 3.
We filtered on Wednesday, and changed and filtered on Monday. 
This was homemade dyes though.
When we switched to Richard Allan we no longer filtered when we changed and then only when a sheen showed up, usually Monday's. The reason was Richard Allan does not require filtering.  


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