[Histonet] looking for method for fixing frozen tissue

Judi Ford jford at cytomx.com
Fri Oct 6 14:34:14 CDT 2017

Hi everyone,
I was wondering if anyone can tell me the best way to formalin fix frozen tissue for processing into a paraffin block. I have tissue in a minus 80 freezer and would like to cut a piece from it to fix in formalin and process. I'm thinking that I could put the tissue into the cryostat and bring it slowly up to minus 20. The container of formalin could be cooled down so that both are near the same temperature when I submerge the tissue into the formalin.  Are there other methods that may help process the tissue better?
Huge thanks to any suggestions that come my way.
Hope you all have a really good weekend.

Judi Gordon
Senior Research Associate
Translational Sciences/IHC
CytomX Therapeutics


The information contained in this electronic message and any attachments to this message are intended for the exclusive use of the addressee(s) and may contain confidential or privileged information. If you are not an intended recipient, or a person responsible for delivering the e-mail to an intended recipient, please be advised that you have received this message in error and that any use, dissemination, forwarding, printing, or copying is strictly prohibited. Please notify the sender at CytomX Therapeutics, Inc., immediately and destroy all copies of this message and any attachments.

CytomX Therapeutics, Inc. <http://www.cytomx.com>

More information about the Histonet mailing list