[Histonet] looking for method for fixing frozen tissue
jford at cytomx.com
Fri Oct 6 14:34:14 CDT 2017
I was wondering if anyone can tell me the best way to formalin fix frozen tissue for processing into a paraffin block. I have tissue in a minus 80 freezer and would like to cut a piece from it to fix in formalin and process. I'm thinking that I could put the tissue into the cryostat and bring it slowly up to minus 20. The container of formalin could be cooled down so that both are near the same temperature when I submerge the tissue into the formalin. Are there other methods that may help process the tissue better?
Huge thanks to any suggestions that come my way.
Hope you all have a really good weekend.
Senior Research Associate
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