[Histonet] Mounting media

[Terri Braud]

Although we've used several brands with good success, our most consistent performer has been the Sakura Tissue Tek Glas Mounting Media #6419. When coverslipping, either automated or manual, the secret to avoiding air bubble during storage is to insure that the correct amount of media is dispensed onto the slide.  If the amount is insufficient, the slide will still coverslip to be read, but as time passes and the xylene dries out, there will be air left under the coverglass which will allow the stain to degrade. When coverslipping by hand, we go by the rule of 3 drops of media from a plastic disposable pipette for a 24x50 No.1 coverglass.  When techs "guesstimate" is when problems occur.  Best of luck, I hope this helps

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal