[Histonet] cytospin prep

[Joe W. Walker, Jr.]

Hi Nina,

How are the cytology specimens collected?  This will play a role in how you process them for evaluation on the Cytospin machine.  We do not utilize the mega funnels.  However, cytology specimens should be centrifuge first to remove any supernatant.  Then equal parts of the Cytospin collection fluid should be added to the cell button and the cell button should be resuspended.  Once resuspended, the amount of specimen that you put into the mega funnel chamber will vary depending on the cellularity of the cytology specimen.  Large cell buttons might require less while small buttons will require more.  If you over load the chamber, you will experience the cell spot/square falling off the slide when staining.  This assumes that you are Pap staining your slides, hence the use of the Cytospin collection fluid.  If you are making airdried specimens for Romanowsky stains, if you over load the chamber, you will have the same result of the spot falling of the slide.  The manufacturer should provide max volume limits for the mega funnels but again a very cellular specimen will create issues.  This has been our experience with cytospins.  

Joe W. Walker, Jr. MS, SCT(ASCP) 
Anatomical Pathology Manager
Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
P 802.747.1790  F 802.747.6525
joewalker at rrmc.org, www.rrmc.org
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-----Original Message-----
From: Nina J. Rich via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Thursday, November 02, 2017 11:38 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] cytospin prep

Good morning,


For all those that have to do cytology cytospins.  We are using the cytospin 4 with the large square mega funnels.  We have a problem with the specimen always washing off the slide.  Is there anyone who can send me their processing procedure for this type of funnel, to include whether or not you centrifuge the specimen first. The small circular funnels do not wash.  Any input would greatly be appreciated.  Jean


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