Perhaps browse the histonet archives for some tips on this staining. I regularly use PMMA stains ( that do not work on glycol). Good Luck. Vicki L. Kalscheur School of Veterinary Medicine Comparative Orthopedic Research Laboratory 2015 Linden Drive Madison, WI 53706-1100 Phone: 608-262-8534 vicki.kalscheur at wisc.edu