[Histonet] Tissue Fixation

Rene J Buesa rjbuesa at yahoo.com
Fri Mar 31 08:32:35 CDT 2017

What you describe as a possible scenario is absolutely possible.If your PT does not "want to hear" about it, suggest she gets a "hearing aid" or to study something about histotechnology or even better yet, pay attention to what a professional on the subject (you) has to say about it. You would never dare to question her diagnosis, why would she question yours on this subject?René 

    On Friday, March 31, 2017 9:11 AM, T H via Histonet <histonet at lists.utsouthwestern.edu> wrote:

 Good Morning,

I have a pathologist that is not happy with the fixation on some of our LEEP specimens.  She swears its histology doing something to the specimen to cause the tissue to look unfixed on only "part" of the LEEP specimens (all the same client specimens).  She claims we must be diluting our formalin to cause this issue or "something".  We mentioned maybe it was on the clients end not placing them in 10% formalin right away, she wouldn't hear of it.

Let me give you some back ground on how our process works.  Our clients send us all our specimens to us via Overnight FedEx or UPS in 10% formalin they will then they sit in 10% formalin in-house until the processors are started around 3pm and sits an additional 5 hours in 10% formalin on the processor before the processor actually starts.  That being said the fixation process has had a pretty good start before we ever even touch it.

My question is, and I thought I had read this in the past is, when a specimen is left out prior to fixation and lying on a absorbent surface such as a paper towel, won't the area of the tissue touching the absorbent surface begin the disintegration processes faster in that exact area then the rest of the specimen?  Or if you have any other suggestion on what might be happening to only "certain" specimens would be great as well.

Thanks for your help!


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