[Histonet] Intensity of Mayer's Hematoxylin

Teri Johnson tejohnson at genoptix.com
Wed Mar 29 12:37:10 CDT 2017

Hi Mariam,

Mayer's is a progressive hematoxylin. Therefore you should increase your time in the stain to get a deeper color. You might try 10 or 20 minutes and see what works best for you.

You can do your bluing in tap water or even buffer solution rather than ammonium hydroxide if you prefer. It's not as fast but potentially more gentle on the tissue.

If you want a more intense blue (be careful you don't obscure light nuclear positivity), you could use a different hematoxylin stain solution.

Best wishes,



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