[Histonet] MITF for Mohs frozen sections

[Trini Nguyen]

Hello all,

Mohs surgeon would like to start doing MITF (microphthalmia transcription
factor) for melanoma with a chromogen yielding red results manually.

I have never done them manually and do not know where to begin. We have a
bond max but it keeps breaking down and you can't stain manually since the
AEC and DAB are mixed by the stainer itself.

Please help with any information and or procedure/protocols you have.