[Histonet] Reference for this Recipe and Use

[Ingles Claire]

Sadly, it is much safer than the old Chloroform clearing method.
Claire

________________________________________
From: Tony Henwood (SCHN) via Histonet [histonet at lists.utsouthwestern.edu]
Sent: Sunday, March 26, 2017 6:22 PM
To: ian bernard
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Reference for this Recipe and Use

This is sometimes known as Newel's solution for revealing lymph nodes in fatty specimens:

Newell KJ, Sawka BW, Rudrick BF, Driman DK. (2001) "GEWF solution" Arch Pathol Lab Med 125:642 645.

Absolute ethanol                        1000ml
Water                                   340ml
Concentrated formalin (37%)             160ml
Glacial acetic acid                     100ml



Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

-----Original Message-----
From: ian bernard via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Saturday, 25 March 2017 3:04 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Reference for this Recipe and Use

500 ML OF 100% ALCOHOL

170 ML OF DISTILLED WATER

80 ML OF 40% FORMALIN

50 ML OF GLACIAL ACETIC ACID



V/R

IB



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